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CHK2, Active

Regulates cell cycle checkpoints and apoptosis
Catalog #: 7712
$295.00

Product Details

Cat # +Size 7712-5
Size 5 μg
Alternate Name CHK, CHK2 checkpoint homolog
Gene Symbol CHK2
Gene ID 11200
Accession # O96017
Source Baculovirus (Sf9 insect cells)
Appearance Liquid
Physical Form Description Recombinant proteins in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).
Molecular Weight 88.0 kDa
Purity by SDS-PAGE ≥90%
Storage Conditions -80°C
Shipping Conditions Dry Ice
USAGE For Research Use Only! Not to be used in humans

Details

Chk2 is the mammalian homolog of the Saccharomyces cerevisiae Rad53 and Schizosaccharomyces pombe Cds1 protein kinases required for the DNA damage and replication checkpoints. Chk2 is rapidly phosphorylated and activated in response to replication blocks and DNA damage; the response to DNA damage occurs in an ataxia telangiectasia mutated (ATM)-dependent manner (1). In vitro, Chk2 phosphorylates p53 on Ser-20 and dissociated preformed complexes of p53 with Mdm2, a protein that targets p53 for degradation (2). In vivo, ectopic expression of wild-type Chk2 leads to increased p53 stabilization after DNA damage, whereas expression of a dominant-negative Chk2 mutant abrogated both phosphorylation of p53 on Ser-20 and p53 stabilization. Thus, in response to DNA damage, Chk2 stabilizes the p53 tumor suppressor protein leading to cell cycle arrest in G1. Chk2 is directly phosphorylated by A™ in response to ionizing radiation (3). The phosphorylation occurs in the Ser-Gln/Thr-Gln (SQ/TQ) cluster domain (SCD) on Chk2, which contains seven SQ/TQ motifs, and Thr68 is the major in vitro phosphorylation site by ATM.


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