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CHK1, Active

based on 1 citations in multiple journalsCHK1, Active14.1 4
Regulates cell cycle checkpoints and apoptosis
Catalog #: 7735

In stock

SKU-Size Size Price Qty
7735-5 5 μg
$295.00
7735-100 100 μg
$3,950.00
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Product Details

Alternate Name CHK, Serine/threonine-protein kinase Chk1, CHK1 checkpoint homolog, Cell cycle checkpoint kinase, Checkpoint kinase-1
Gene Symbol CHK1
Gene ID 1111
Accession # O14757
Source Baculovirus (Sf9 insect cells)
Appearance Liquid
Physical Form Description Recombinant protein in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 30% glycerol.).
Molecular Weight 82.0 kDa
Purity by SDS-PAGE ≥90%
Handling Centrifuge the vial prior to opening.
Storage Conditions -80°C
Shipping Conditions Dry Ice
USAGE For Research Use Only! Not to be used in humans

Details

CHK1 is a 56 kd serine/threonine protein kinase that was originally identified in fission yeast to play a role in activation of the DNA damage checkpoint in the G2 phase of the cell cycle (1). CHK1 appears to function downstream of several of the known fission yeast checkpoint gene products, including that encoded by rad3+, a gene with sequence similarity to the A™ gene mutated in patients with ataxia telangiectasia (2). In vitro, CHK1 binds to and phosphorylates the dual-specificity protein phosphatases Cdc25A, Cdc25B, and Cdc25C, which control cell cycle transitions by dephosphorylating cyclin-dependent kinases (3). CHK1 phosphorylates Cdc25C on serine-216 which creates a binding site for 14-3-3 protein and inhibits function of the phosphatase. Thus, in response to DNA damage, CHK1 phosphorylates and inhibits Cdc25C, thereby preventing the activation of the Cdc2-cyclin B complex and mitotic entry.


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Larsen B. et. al. Caspase 3/caspase-activated DNase promote cell differentiation by inducing DNA strand breaks PNAS, Mar 2010; 107: 4230 - 4235.
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