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cGMP Direct Immunoassay Kit (Colorimetric)

based on 3 citations in multiple journalscGMP Direct Immunoassay Kit (Colorimetric)34.1 4
Highly Sensitive Assay
Catalog #: K372
$425.00

Product Details

Cat # +Size K372-100
Size 100 assays
Detection Method Absorbance (450 nm)
Species Reactivity Mammalian
Applications The kit can detect 0.04 -10 pmol/well (0.008 - 2 µM) cGMP samples.
Features & Benefits • Simple procedure
• Fast and convenient
• The assay is sensitive, stable and high-throughput adaptable
• The kit provides a new acetylation procedure to improve detection sensitivity significantly
Kit Components • 10X cGMP Assay Buffer
• Standard cGMP (10 nmol)
• Neutralizing Buffer
• Acetylating Reagent A
• Acetylating Reagent B
• Anti-cGMP pAb/BSA
• Protein G Coated Plate
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Adenosine and guanosine 3’,5’-cyclic monophosphate (cAMP and cGMP) are important “second messengers” involved in many physiological processes. BioVision’s cGMP Direct Immunoassay Kit provides a direct competitive immunoassay for sensitive and quantitative determination of cGMP level in biological samples. The kit utilizes the recombinant Protein G coated plate to anchor cGMP polyclonal antibody. cGMP-HRP conjugates directly competes with cGMP from samples for binding to the cGMP specific antibody on the plate. After incubation and washing, the amount of cGMP-HRP bound to plate can easily be determined by reading OD450 nm. The intensity of OD450 nm is inversely proportional to the concentration of cGMP in samples. The kit provides a new acetylation procedure that improves detection signal significantly. The kit can detect 0.04 -10 pmol/well (0.008 - 2 µM) cGMP samples.


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Guanosine 3,5-cyclic monophosphate (cyclic GMP, cGMP) has been shown to be present at levels typically 10-100 fold lower than cAMP in most tissues. How does this fit with the cAMP Direct Immunoassay Kit to be much more sensitive, 1~100 fmol of cAMP per assay, than the cGMP Direct Immunoassay Kit, 0.1-10 pmol cGMP per assay?
The cAMP antibody affinity is higher than that of cGMP. That is the reason why you need fmol of cAMP while for cGMP you need pmol quantities.
Would the release of hemoglobin from lysed RBC interfere with the read-out?
The hemoglobin will not bind to the antibody in the plate. It will go out with the wash.
How stable is cGMP in 0.1 M HCl?
cGMP is very stable in 0.1 M HCl.
Can I freeze my cell lysate at -70 °C and test the cGMP levels later?
Yes
How much sample do we need?
10 Million cells or 100 mg of tissue
How does the plotting of the standard curve works?
The Abs is reverse proportionally to cGMP dose, so Abs of 10 pmol/ul is lower than the 0 pmol/ul. Acetylation step increase signal very significantly. Stop the reaction by adding 100ul 1M HCl may also double the signal.
Sollinger et al., Damage-associated molecular pattern activated Toll-like receptor 4 signalling modulates blood pressure in L-NAME-induced hypertension.Cardiovasc Res, Mar 2014; 101: 464 - 472.
Sollinger et al., Damage-associated molecular pattern activated Toll-like receptor 4 signalling modulates blood pressure in L-NAME-induced hypertension. Cardiovasc Res,  Jan 2014; 10.1093/cvr/cvt265.
Zhang F et al. (2008) Ann. Thorac. Surg. 85: 581 - 585.
For more citations of this product click here