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CETP Inhibitor Screening Kit (Fluorometric) II

The most advanced kit on the market - includes human CETP enzyme and CETP inhibitor Anacetrapib
Catalog #: K594

In stock

$555.00

Product Details

Cat # +Size K594-100
Size 100 assays
Detection Method Fluorescence (Ex/Em 480/511 nm)
Species Reactivity Mammalian
Applications Screening/studying/characterizing CETP inhibitors
Features & Benefits • The most advanced kit on the market - includes human CETP enzyme and CETP inhibitor Anacetrapib for ultimate user-convenience.
• Simple and sensitive assay
• High-Throughput adaptable
Kit Components • CETP Assay Buffer
• Donor Molecule
• Acceptor Molecule
• Enriched Human CETP
• Inhibitor (Anacetrapib, 1 mM)
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Cholesteryl ester transfer protein (CETP) is a plasma protein that transfers a cholesteryl ester from HDL to LDL or VLDL in exchange for a triglyceride. HDL plays an important role in lipid metabolism and cardiovascular health. HDL transports cholesterol to the liver for excretion or to steroidogenic tissues for steroid synthesis. HDL also plays an important role in the reverse cholesterol transport pathway, removing cholesterol from lipid-filled macrophages, protecting against atherosclerosis. Because of this function, CETP is viewed as a target to increase HDL, with CETP inhibition being an active area of research and several CETP inhibitors at various stages of drug development. BioVision’s CETP Inhibitor Screening Kit uses a self-quenched fluorescent neutral lipid that can be measured when transferred to an acceptor molecule. The fluorometric intensity is directly proportional to the amount of neutral lipid transfer. Enriched Human CETP from plasma is included for screening. A CETP inhibitor (Anacetrapib) is included as a positive control.


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Is the chloride reagent supposed to come in bright purple color? or should it be rather colorless?
The chloride reagent is bluish in colour to begin with. The final colour developed is going to be intense blue which can be detected at 620 nm.
Can this kit be used with samples like bacteria, plants, drosophila, yeast etc?
We have optimized the kit with mammalian samples. However, theoretically these kits should work with samples from multiple species/sources. Since the optimal conditions depend on the sample type, the protocol has to be be adapted to fit the samples for efficient results. Please refer to this kit's citations to see what kind of samples have been used with this kit other than mammalian samples.
Can we use frozen samples with this assay?
Fresh samples are always preferred over frozen samples. However, frozen samples can also be used, provided, they were frozen right after isolation, were not freeze thawed multiple time (for which we recommend aliquoting the samples before freezing) and have been frozen for relatively short periods.
Can we use a different wavelength than recommended for the final analysis?
It is always recommended to use the exact recommended wavelength for the most efficient results. However, most plate readers have flexibility in their band width of detection in increments of +/- 10 nm. Depending on this flexibility range, you can deviate from the recommended wavelengths within limits.
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact yopur regional BioVision distributor.
What is the shelf life of this kit?
This kit is good for 12 months from the date of shipment in the unopened form when stored at the appropriate temperature and appropriate conditions. After opening and reconstitution, some of the components in this kit are good for 2 months at -20°C. Please refer to the datasheet for storage information and shelf life of each of the components.
Why are my standard curve values lower than those shown on the datasheet?
There are multiple factors which influence the signals like the incubation times, room temperature, handling etc. In general, to increase the value of the standards, you can increase the incubation time. As long as the standard curve is linear, it should be fine to use, since all of your samples will also be measured under the same conditions on this curve.
How do I normalize my samples against protein concentration
You can use a protein quantitation assay on the supernatants you get from cell/tissue lysates or with any other liquid sample in the assay buffer.
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.
Should I make a standard curve for every expt I do, or is one curve/kit enough?
Yes, I would strongly recommend you to do the standards every time you do the expt. There is always a chance that something was done differently that day and we do not want any conditions to differ between standards and samples.
About the measurement, could my customer uses a end-point way to using this kit ?
Kinetic analysis allows to measure inhibition curves for IC50 calculation. Measuring one time point does not allow that. Each inhibitor works at a different time scale and to compare them, it is essential to get data kinetically.
There is a vial of Enriched Human CETP in this kit , how much proteins in it ? ( ug ? or units ? )
The enriched CETP provided is from pooled human plasma and hence the exact amount cannot be shared, there is lot to lot variation. The same amount is added into each well during inhibitor screening using each kit.