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Cell Transformation Assay Kit (Fluorometric)

Highly Sensitive and Quantitative (Fluorometric), HTP adaptable Assay to measure Cell Transformation
Catalog #: K922
$505.00

Product Details

Cat # +Size K922-100
Size 100 assays
Detection Method Fluorescence (Ex/Em = 480/530 nm)
Species Reactivity Mammalian cells
Applications • Measure cell transformation in response to stimuli
• Screen and characterize compounds that influence cell transformation
Features & Benefits • Highly sensitive fluorometric method to measure cell transformation in response to a variety of biochemical stimuli
• Simple & High throughput-adaptable
• Reproducible, Quantitative tool for screening, studying, and characterizing compounds that affect cell transformation
Kit Components • Agarose Powder
• DMEM Solution (10X)
• Staining Solution
• Agarose Solubilization Solution
• Quantitative Dye (200X)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Transformed cells can proliferate without attaching to surface Anchorage-independent cell growth is the hallmark of cell transformation. The Soft-Agar Assay is a traditional method for screening cell transformation in vitro. However, this method is lengthy (3-4 weeks incubation), laborious (counting colonies) and inconsistent (due to subjective counting). BioVision’s Cell Transformation Assay is faster, stable, and more sensitive than the traditional soft-agar assay. The kit uses a quantitative dye that binds to nucleic acid and generates green fluorescence. This one-step method is non-radioactive and simple (just add-and-read, and does not require tedious labor such as counting colonies). The assay is high-throughput adaptable and has wide linear range from 50-60,000 cells. The entire assay can be finished within 7-8 days.


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