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Cell Proliferation Assay Kit (Fluorometric)

Non-radioactive Assay, detects as less as 25-60,000 cells
Catalog #: K307

Product Details

Cat # +Size K307-1000
Size 1000 assays
Detection Method Fluorescence (Ex/Em = 480/538 nm)
Applications Measurement of cell proliferation in response to growth factors, cytokines, mitogens, and nutrients
Analysis of cytotoxic and cytostatic compounds such as anticancer drugs, toxic agents, and other pharmaceuticals
Assessment of physiological mediators and antibodies that inhibit cell growth
Features & Benefits • Convenient (just add-and-read)
• Non-radioactive
• High-throughput
• Wide linear range detection: 25-60,000 cells
• Suitable for cell proliferation, and cytotoxicity
Kit Components • Cell Lysis Buffer (5X)
• Nuclear Dye (200X)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


BioVision’s Cell Proliferation Assay Kit (Fluorometric) provides an easy and quantitative method to measure cell proliferation and cytotoxicity. The assay is based on a nuclear dye that specifically binds to nucleic acid in the cell and generates green fluorescence. The generated fluorescent intensity is directly proportional to the cell number, which can be quantified by measuring fluorescence (Ex/Em = 480/538 nm). This assay kit provides a just add-and-read, non-radioactive, and high-throughput method for cell proliferation and apoptosis. The assay is rapid and more sensitive than MTT, XTT, or MTS-based assays and can detect a wide linear range of 25-60,000 cells.

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Can the kit work on bacteria or yeast cells?
The kit has been standardized for mammalian cells only
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact your regional BioVision distributor.
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.
Monica Ceccon, Mitochondrial Hyperactivation and Enhanced ROS Production are Involved in Toxicity Induced by Oncogenic Kinases Over-Signaling. Cancers (Basel), Dec 2018;  30545064.
Yunjie Xu, et al., (2018) ABT737 reverses cisplatin resistance by targeting glucose metabolism of human ovarian cancer cells, International Journal of Oncology, Jun. 2018, 30015875
For more citations of this product click here