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CDCA8 (Human) ELISA Kit

A Sandwich ELISA kit for the quantitative measurement of CDCA8 in human plasma, tissue homogenates and other biological fluids.
Catalog #: E4813
$725.00

Product Details

Cat # +Size E4813-100
Size 96 assay
Detection Method Sandwich ELISA, Absorbance (450 nm)
Species Reactivity Human
Applications • A Sandwich ELISA kit for the quantitative measurement of CDCA8 in human plasma, tissue homogenates and other biological fluids.
Features & Benefits • This ELISA kit is used for in vitro quantitative determination of Human CDCA8.
• Detection Range: 0.156-10 ng/ml
• Sensitivity: 0.094 ng/ml
• Precision: Intra-Assay: CV<8%, Inter-Assay: CV<10%
Kit Components • Micro ELISA Plate
• Lyophilized Standard (10 ng)
• Sample / Standard dilution buffer
• Biotin- labeled antibody
• Antibody dilution buffer
• HRP-Streptavidin Conjugate (SABC)
• SABC dilution buffer
• TMB substrate
• Stop Solution
• Wash buffer (25X)
• Plate sealers
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

CDCA8 gene encodes a component of the chromosomal passenger complex. This complex is an essential regulator of mitosis and cell division. This protein is cell-cycle regulated and is required for chromatin-induced microtubule stabilization and spindle formation. Some studies suggests that CDCA8 is a key mediator of estrogen-stimulated breast cancer cell growth and survival, which can be utilized as a novel target in breast cancer treatment. BioVision’s CDCA8 (Human) ELISA Kit is based on the Sandwich ELISA principle. Capture antibody was precoated onto 96-well micro ELISA plate and the biotin conjugated antibody was used as detection antibody. The standards, test samples, and biotin conjugated detection antibody were added to the wells subsequently and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding the acidic stop solution. The density of color is proportional to the amount of CDCA8 captured from the samples.


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