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CD-45 Antibody (CT)

Polyclonal Antibody to detect CD-45 in human samples
Catalog #: 6734
$245.00

Product Details

Cat # +Size 6734-100
Size 100 μl
Antibody Target CD-45
Alternate Name PTPRC; CD45; Receptor-type tyrosine-protein phosphatase C; Leukocyte common antigen; T200; Flags: Precursor.
Host Rabbit
Antibody Type Polyclonal
Isotype Rabbit IgG
Immunogen This CD45 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1245-1275 amino acids from the C-terminal region of human CD45.
Accession # P08575
Gene ID 5788
Appearance Colorless liquid
Formulation 100 µl of antibody in PBS with 0.09% (W/V) sodium azide
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Species Reactivity Human
Application Western blot, IHC, FACS
Positive Control Western blot: Jurkat cell lysate, IHC: human tonsil tissue, FACS: Jurkat cells
Application & Usage Western blot: ~1:1000, IHC: ~1:10–1:50, FACS: ~1:10-1:50.
Handling The antibody solution should be gently mixed before use.
Storage Conditions -20 °C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

CD45 has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45 are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5 and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45, while the intracellular domain is conserved. CD45 functions as a phosphotyrosine phosphatase, a vital component for efficient tyrosine phosphorylation induction by the TCR/CD3 complex. The tyrosine phosphatase activity of CD45 is contained within the conserved intracellular domain. Src and Syk family protein tyrosine kinases are utilized by the TCR/CD3 complex to initiate signaling cascades. Several members of these two families, including Lck, Fyn and ZAP-70, have been implicated as physiological substrates of CD45.


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