Cathepsin S Activity Fluorometric Assay Kit

Catalog #: K144 | abID: ab65307

Product Details

abID ab65307
Cat # +Size K144-100
Size 100 assays
Detection Method Fluorescence (Ex/Em 400/505)
Species Reactivity Mammalian
Applications Detects alternative proteolytic enzymes such as the lysosomal cathepsin proteases that can initiate or propagate proapoptotic signals.
Features & Benefits • Simple one-step procedure; takes only 1-2 hours
• Fast and convenient
• Cathepsin-S assay is straightforward, and can be adapted to 96-well plate assays.
Kit Components • CS Cell Lysis Buffer
• CS Reaction Buffer
• CS Substrate Z-VVR-AFC (10 mM)
• CS Inhibitor (1 mM)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Apoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals. Cathepsins are lysosomal enzymes that are also used as sensitive markers in various toxicological investigations. The Cathepsin-S Activity Assay kit is a fluorescence-based assay that utilizes the preferred cathepsin-S substrate sequence VVR labeled with AFC (amino-4-trifluoromethyl coumarin). Cell lysates or other samples that contain cathepsin-S will cleave the synthetic substrate Z-VVR-AFC to release free AFC. The released AFC can easily be quantified using a fluorometer or fluorescence plate reader.

Why buy BioVision Products?
Global Presence
Technical Support
BioVision aims to provide our customers innovative tools for accelerating drug discovery and biological research. BioVision offers >8,000 products including the most comprehensive array of assay kits for key targets in Metabolic pathways.
BioVision is committed to providing the highest quality products at a competitive price.
We have a broad network of global distributors who are ready to address your research needs and ensure fast delivery.
Our highly trained Technical Support team provides comprehensive product support and is dedicated to resolving your issues quickly and efficiently.
How should tissue lysates be prepared?
We have never tested this kit on tissue samples. However, if you want to test, you could homogenize the tissue in Cell Lysis buffer, preferably using a Dounce homogenizer. Centrifuge the lysate and use the supernatant for the assay.
Will this kit work with mouse or rat samples?
This kit is compatible with all mammalian samples including mouse and rat.
Can we interchange the buffer from this kit with K143?
Each buffer is of different composition and they cannot be used
Can the kit work on bacteria or yeast cells?
The kit has been standardized for mammalian cells only
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact yopur regional BioVision distributor.
What is the shelf life of this kit?
The expiry date is typically 1 year from the date of shipment
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.
I am planning to use the Lowry method to determine proteins in my sample. Is the Cell Lysis Buffer provided with the kit compatible with Lowry method?
K144 CS Cell Lysis Buffer is compatible with Lowry method as the EDTA level and detergent level are both within the acceptable range of Lowry method for protein estimation.
Wannita Klinngam, Cathepsin S Alters the Expression of Pro-Inflammatory Cytokines and MMP-9, Partially through Protease—Activated Receptor-2, in Human Corneal Epithelial Cells. Int J Mol Sci, Nov 2018;  30423938.
Chanyang Kim, et al., (2018) IGF-1 protects SH-SY5Y cells against MPP+-induced apoptosis via PI3K/PDK-1/Akt pathway, Endocrine Connections, Jun. 2018, 29459421
McComb et al., Cathepsins Limit Macrophage Necroptosis through Cleavage of Rip1 Kinase.J. Immunol., Jun 2014; 192: 5671 - 5678.
Chang C-J et al (2009) Am. J. Pathol.; 174: 1837 - 1846.
Taleb, S., et al. (2006) J. Clin. Endocrinol. Metab. 91: 1042-1047.
For more citations of this product click here