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Product Details
| Cat # +Size | K147-100 |
|---|---|
| Size | 100 assays |
| Detection Method | Fluorescence (Ex/Em = 400/505 nm) |
| Applications | Screen/study/characterize potential inhibitors of Cathepsin B. |
| Features & Benefits | • Simple & convenient • High-throughput adaptable assay to screen/study/characterize the potential inhibitors of Cathepsin B • Cathepsin B inhibitor, F-F-FMK is included in the kit |
| Kit Components | • CTSB Reaction Buffer • CTSB Reagent • Cathepsin B (human) • CTSB Substrate, Ac-RR-AFC (10 mM) • CTSB Inhibitor (F-F-FMK, 1 mM) |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
Cathepsin B (CTSB, EC 3.4.22.1) is a lysosomal cysteine proteinase that is suggested to participate in intracellular degradation and turnover of proteins. It has also been implicated in tumor invasion and metastasis. Biovision’s Cathepsin B Inhibitor Screening Kit utilizes the ability of Cathepsin B to cleave the synthetic AFC based peptide substrate to release AFC, which can be easily quantified using a fluorometer or fluorescence microplate reader. In the presence of a Cathepsin B-specific inhibitor, the cleavage of the substrate is reduced/abolished resulting in decrease or total loss of the AFC fluorescence. This simple and high-throughput adaptable assay kit can be used to screen/study/characterize the potential inhibitors of Cathepsin B.
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How should tissue lysates be prepared?
Samples should be completely homogenized in the assay buffer, preferably using a Dounce homogenizer. Substances such as EDTA (> 0.5mM), ascorbic acid (> 0.2%), sodium azide (> 0.2%), NP-40 and Tween-20 (>1%) are known to interfere with these assays and should be avoided in sample preparation.
Will this kit work with mouse or rat samples?
This kit is compatible with all mammalian samples including mouse and rat.
Can we interchange the buffer from this kit with K143?
Each buffer is of different composition and they cannot be used
Can the kit work on bacteria or yeast cells?
The kit has been standardized for mammalian cells only
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact yopur regional BioVision distributor.
What is the shelf life of this kit?
The expiry date is typically 1 year from the date of shipment
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.
