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CaspSELECT™ Caspase-3 Immunoassay Kit (Fluorometric)

Specifically detects Caspase-3
Catalog #: K163
$645.00

Product Details

Cat # +Size K163-100
Size 100 assays
Detection Method Fluorescence (Ex/Em 370-425/ 490-525 nm)
Species Reactivity Mammalian
Applications Effective immunosorbent enzyme assay for specific, quantitative detection of caspase-3 activity in microtiter plates.
Features & Benefits • Simple one-step procedure; takes only ~3 hours
• Fast and convenient
Kit Components • Cell Lysis Buffer
• Coating Buffer
• Anti-Caspase-3 Antibody (20X)
• Blocking Buffer
• Incubation Buffer
• DTT (1 M)
• DEVD-AFC Substrate (1 mM)
• Positive Control (rh-Caspase-3)
• Microtiter Plate
• Adhesive Plate Cover
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Activation of caspase-3 plays a key role in initiation of cellular events during apoptosis. The CaspSELECT™ Caspase-3 Immunoassay Kit provides an effective immunosorbent enzyme assay for specific, quantitative detection of caspase-3 activity in microtiter plates. The assay utilizes caspase-3 polyclonal antibody to capture activated caspase-3 from cell lysates. Caspase substrate DEVD-AFC is then added and is cleaved proportionally to the amount of activated caspase-3 in the cell lysate. The cleavage generates free AFC which can be analyzed fluorometrically (Ex./Em. = 400 nm/505 nm) using a fluorescence plate reader. The assay ensures absolute specific detection of caspase-3. Other known caspases and non-specific proteases are not detected.


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