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Product Details
| Detection Method | Fluorescence microscopy, flow cytometry, or fluorescence plate reader. |
|---|---|
| Species Reactivity | Mammalian |
| Applications | For detecting activated caspase-3/7 in living cells |
| Features & Benefits | • The green fluorescence label allows for direct detection of activated caspases in living cells
• Fast and convenient • The caspase inhibitor, Z-VAD-FMK, is included in the kit as an additional control |
| Kit Components | • Green-DEVD-FMK
• Wash Buffer • Z-VAD-FMK |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
Activation of caspases plays a central role in apoptosis. The CaspGLOW™ Green Active Caspase-3/7 Staining Kit provides a convenient means for detecting activated caspase-3/7 in living cells. The assay utilizes the caspase inhibitor DEVD-FMK conjugated to FITC (Green-DEVD-FMK) as the fluorescent in situ marker. Green-DEVD-FMK is cell permeable, nontoxic, and irreversibly binds to the activated caspase-3/7 in apoptotic cells. The green fluorescence label allows the direct detection of activated caspase-3/7 in apoptotic cells by fluorescence microscopy, flow cytometry, or fluorescence plate reader. The caspase inhibitor, Z-VAD-FMK, is included in the kit as an additional control. It can be used to inhibit caspase activation in an induced culture.
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