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CaspGLOW™ Fluorescein Active Caspase Staining Kit

based on 14 citations in multiple journalsCaspGLOW™ Fluorescein Active Caspase Staining Kit145 5
Detects activated Caspases by FACS, FL or plate reader
Catalog #: K180
SKU-Size Size Price Qty
K180-25 25 assays
$170.00
K180-100 100 assays
$460.00
More Sizes Get Quote

Product Details

Detection Method Fluorescence microscopy, Flow cytometry and fluorescence plate reader (Ex. = 485 nm and Em. = 535 nm)
Species Reactivity Mammalian
Applications Sensitive detection of activated caspases in living cells.
Features & Benefits • Simple one-step procedure; takes only 1-2 hours
• Fast and convenient
• The FITC label allows for direct detection of the activated caspases in apoptotic cells
Kit Components • FITC-VAD-FMK
• Wash Buffer
• Z-VAD-FMK
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Activation of caspases plays a central role in apoptosis. The CaspGLOW™ Fluorescein Active Caspase Staining Kit provides a convenient and sensitive means for detecting activated caspases in • Living cells. The assay utilizes the caspase family inhibitor VAD-FMK conjugated to FITC (FITC-VAD-FMK) as a marker. FITC-VAD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspases in apoptotic cells.


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How should cells be removed from the culture dish- by trypsin or lysate buffer?
K180 is for staining active caspases in live cells, so there is no lysis required. After washing at step 6, collect cells by trypsinization. Centrifuge cells at 3000 rpm for 5 minutes and remove supernatant. Resuspend cells in 0.5 ml of PBS or Wash Buffer, and proceed with flow cytometry analysis. Be careful and do not over trypsinize.
Could you let me know whether you have used baculovirus to produce this product?
None of the kit components have been made using baculovirus
Do different cell lines react differently or require different probe/tracer concentrations?
Yes. Some cell lines are more prone to high background levels of apoptosis, other cell lines require longer incubation periods and longer (and more thorough) wash steps due to decreased cell permeability.
Can the kit work on bacteria or yeast cells?
The kit has been standardized for mammalian cells only
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact yopur regional BioVision distributor.
What is the shelf life of this kit?
The expiry date is typically 1 year from the date of shipment
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.
Lee, Ji Young et al. (2017) Pro-Apoptotic Role of the Human YPEL5 Gene Identified by Functional Complementation of a Yeast moh1Δ Mutation, J Microbiol Biotechnol. 2017 Mar 28;27(3):633-643.
Vogel, Katharina U. et al. (2016) The RNA-Binding Proteins Zfp36l1 and Zfp36l2 Enforce the Thymic β-Selection Checkpoint by Limiting DNA Damage Response Signaling and Cell Cycle Progression, J Immunol. 2016 Oct 1;197(7):2673-85.
Vomhof-DeKrey et al., Cognate interaction with iNKT cells expands IL-10–producing B regulatory cells. PNAS, Oct 2015; 112: 12474 - 12479.
Li et al., Identification of miR-133b and RB1CC1 as Independent Predictors for Biochemical Recurrence and Potential Therapeutic Targets for Prostate Cancer. Clin. Cancer Res., May 2014; 20: 2312 - 2325.
Désio Aurélio Farias-de-Oliveira et al., Caspase-8 and caspase-9 mediate thymocyte apoptosis in Trypanosoma cruzi acutely infected mice. J. Leukoc. Biol., Feb 2013; 93: 227 - 234
For more citations of this product click here