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CaspGLOW™ Fluorescein Active Caspase-2 Staining Kit

based on 6 citations in multiple journalsCaspGLOW™ Fluorescein Active Caspase-2 Staining Kit64.2 4
Detects activated Caspase-2 by FACS, FL or plate reader
Catalog #: K182
SKU-Size Size Price Qty
K182-25 25 assays
$170.00
K182-100 100 assays
$435.00
More Sizes Get Quote

Product Details

Detection Method Fluorescence microscopy, Flow cytometry and fluorescence plate reader (Ex. = 485 nm and Em. = 535 nm)
Species Reactivity Mammalian
Applications Sensitive detection of activated caspase-2 in living cells.
Features & Benefits • Simple one-step procedure; takes only 1-2 hours
• Fast and convenient
• The FITC label allows for direct detection of the activated caspases in apoptotic cells
Kit Components • FITC-VDVAD-FMK
• Wash Buffer
• Z-VAD-FMK
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Activation of caspases plays a central role in apoptosis. The CaspGLOW™ Fluorescein Active Caspase-2 Staining Kit provides a convenient means for sensitive detection of activated caspase-2 in • Living cells. The assay utilizes the caspase-2 inhibitor, VDVAD-FMK, conjugated to FITC (FITC-VDVAD-FMK) as a marker. FITC-VDVAD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase-2 in apoptotic cells.


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Can you use this kit to stain adherent cells?
The cells should be trypsinized first and then use the protocol for cells in suspension.You can apply the apoptosis-inducing treatment to the cells in suspension after trypsinization.
How soon should stained cells be analyzed to prevent photo bleaching?
Keep samples in the dark or cover them with tin foil. They can be read any time after the final wash, probably up to 3 hours with no noticeable photo-bleaching. You do not want to expose them to high intensity scope illumination such as UV light (which may induce photo-bleaching).
Do different cell lines react differently or require different probe/tracer concentrations?
Yes. Some cell lines are more prone to high background levels of apoptosis, other cell lines require longer incubation periods and longer (and more thorough) wash steps due to decreased cell permeability.
Can the kit work on bacteria or yeast cells?
The kit has been standardized for mammalian cells only
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact yopur regional BioVision distributor.
What is the shelf life of this kit?
The expiry date is typically 1 year from the date of shipment
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.
Raquel Espín et al., TNF receptors regulate vascular homeostasis in zebrafish through a caspase-8, caspase-2 and P53 apoptotic program that bypasses caspase-3. Dis. Model. Mech., Mar 2013; 6: 383 - 396.
Ito, E. et al. Potential Use of Cetrimonium Bromide as an Apoptosis-Promoting Anticancer Agent for Head and Neck Cancer. Mol. Pharmacol. 2009; 76: 969-983.
Hui ABY et al (2009) Clin. Cancer Res.; 15: 3716 - 3724.
Alajiz N et al (2008) Clin. Cancer Res. 14: 4891 - 4897.
Abdel-Latif, L. et al. (2006) J. Gen. Virol. 87: 2539-1548.
For more citations of this product click here