Product Details
| Alternate Name | Ac-LEHD-AFC, Caspase-9 Substrate, Fluorogenic |
|---|---|
| Peptide Sequence | Ac-Leu-Glu-His-Asp-AFC |
| Appearance | Liquid |
| Formulation | DMSO ( 1 mM) |
| Molecular Formula | C₃₃H₃₈F₃N₇O₁₁ |
| Molecular Weight | 765.7 |
| Purity | >98% by HPLC |
| SMILES | CC(C)CC(C(=O)NC(CCC(=O)O)C(=O)NC(CC1=CN=CN1)C(=O)NC(CC(=O)O)C(=O)NC2=CC3=C(C=C2)C(=CC(=O)O3)C(F)(F)F)NC(=O)C |
| InChi | InChI=1S/C33H38F3N7O11/c1-15(2)8-22(39-16(3)44)31(52)41-21(6-7-26(45)46)29(50)42-23(9-18-13-37-14-38-18)32(53)43-24(12-27(47)48)30(51)40-17-4-5-19-20(33(34,35)36)11-28(49)54-25(19)10-17/h4-5,10-11,13-15,21-24H,6-9,12H2,1-3H3,(H,37,38)(H,39,44)(H,40,51)(H,41,52)(H,42,50)(H,43,53)(H,45,46)(H,47,48) |
| InChi Key | HULKIXRFKRCRHD-UHFFFAOYSA-N |
| PubChem CID | 44134612 |
| MDL Number | MFCD01862610 |
| Handling | Protect from light and moisture |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
Ready-to-use fluorometric substrate for caspase-9/Mch-6 and related caspases that recognize the amino acid sequence LEHD. Caspase activity can be quantified by fluorescent detection of free AFC after cleavage from the peptide substrate LEHD-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized, using a hand-held long-UV lamp. The ready-to-use caspase substrate provides an economic alternative for researchers who perform large amount of caspase assays. Cell Lysis Buffer (Cat. #1067-100, -400) and 2X Reaction Buffer (Cat. #1068-20, -80) and DTT (Cat.# 1201-1) for caspase assays are also available separately.
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Ray, D. M., et al. (2005) J. Immunol. 174: 4060-4069.
Yano, T., et al. (2005) Am. J. Pathol. 166:1333-1342.
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