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Caspase-9 Inhibitor Drug Screening Kit (Fluorometric)

based on 3 citations in multiple journalsCaspase-9 Inhibitor Drug Screening Kit (Fluorometric)34.1 4
Catalog #: K159

Product Details

Cat # +Size K159-100
Size 100 assays
Detection Method Fluorescence (Ex/Em 400/505 nm)
Species Reactivity Mammalian
Applications Effective means for screening caspase inhibitors using fluorometric methods.
Features & Benefits • Simple one-step procedure; takes only 1-1.5 hours
• Fast and convenient
• Inhibition of caspase-9 activity can be determined by comparison of the fluorescence intensity in samples with and without the testing inhibitors.
Kit Components • 2X Reaction Buffer
• Caspase Substrate LEHD-AFC (1 mM)
• DTT (1 M)
• Active Caspase-9 (Lyophilized)
• Caspase Inhibitor, Z-VAD-FMK (2 mM)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Caspases have been shown to play a crucial role in apoptosis induced by various deleteriousand physiologic stimuli. Inhibition of caspases can delay apoptosis, implicating a potentialrole in drug screening efforts. The Caspase-9 Inhibitor Drug Screening Kit provides aneffective means for screening caspase inhibitors using fluorometric methods. The assayutilizes synthetic peptide substrate LEHD-AFC (AFC, 7-amino-4-trifluoromethyl coumarin).Active caspase-9 cleaves the synthetic substrate to release free AFC which can then bequantified by fluorometry. Compounds to be screened can directly be added to the reactionand the level of inhibition of caspase-9 activity can be determined by comparison of thefluorescence intensity in samples with and without the testing inhibitors. The assay is simple,straightforward, and can be performed directly in microtiter plates. Each kit contains 100 unitsof active caspase-9, sufficient for screening 100 caspase inhibitor samples. Assay conditionshave been optimized to obtain the maximal activity.

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Do the protease inhibitor that I added will interfere with the assay?
Protease inhibitors will interefere with the assay. Caspase 9 is a cysteine protease and can be inhibited by most protease inhibitor cocktails. The assay is based on the catalytic clevage of the substrate DEVD-pNa by Caspase 9. Inhibition of caspase 9 would thus interfere with the measurement.
Can you test plasma samples?
The kit is good for cell and tissue lysates only and not for plasma samples.
What is the sensitivity range?
The colorimetric substrate has a sensitivity range >1uM.
Will this kit detect full length caspase 9 or cleaved caspase 9?
This kit will detect active Caspase 9 from samples. The procaspase 9 (inactive) is proteolytically processed into the active Caspase 9. I hope this information helps.
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact yopur regional BioVision distributor.
What is the shelf life of this kit?
The expiry date is typically 1 year from the date of shipment
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.
Teachey D.T. et al. (2006) Blood 108: 1965-1971.
Wallach-Dayan S. et al. (2006) Am. J. Physiol. Lung Cell Mol. Physiol. 290: L790-L796.
Wei Y. et al. (2006) Am. J. Physiol. Endocrinol. Metab. E 275-E281.
For more citations of this product click here