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Caspase-8 Substrate IETD-AFC

based on 14 citations in multiple journalsCaspase-8 Substrate IETD-AFC145 4.6
A Caspase-8 substrate
Catalog #: 1062
SKU-Size Size Price Qty
1062-200 200 assays
1062-1000 1000 assays
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Product Details

Alternate Name Ac-IETD-AFC, Caspase-8 Substrate, Fluorogenic
Peptide Sequence Ac-Ile-Glu-Thr-Asp-AFC
Appearance Liquid
Formulation DMSO (2 mM)
Molecular Formula C₃₁H₃₈F₃N₅O₁₂
Molecular Weight 729.7
Purity >95% by HPLC
InChi InChI=1S/C31H38F3N5O12/c1-5-13(2)25(35-15(4)41)29(49)37-19(8-9-22(42)43)27(47)39-26(14(3)40)30(50)38-20(12-23(44)45)28(48)36-16-6-7-17-18(31(32,33)34)11-24(46)51-21(17)10-16/h6-7,10-11,13-14,19-20,25-26,40H,5,8-9,12H2,1-4H3,(H,35,41)(H,36,48)(H,37,49)(H,38,50)(H,39,47)(H,42,43)(H,44,45)/t13-,14+,19-,20-,25-,26-/m0/s1
PubChem CID 25108570
Handling Protect from light and moisture
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Ready-to-use fluorometric substrate for FLICE/caspase-8 and related caspases that recognize the amino acid sequence IETD. The sequence IETD is based on caspase-8 cleavage site in CPP32/caspase-3 proenzyme. FLICE and related caspase activity can be quantified by fluorescent detection of free AFC after cleavage from the peptide substrate IETD-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized using a hand-held long-UV lamp.

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Bok K et al (2009) J. Virol.; 10.1128/JVI.02028-08.
Ghosh, S., et al. (2005) Am. J. Physiol Heart Circ Physiol. 289: H768-H776.
Ghosh, S., et al. (2005) Am. J. Physiol Heart Circ Physiol. 289: H768-H776.
Ray, D. M., et al. (2005) J. Immunol. 174: 4060-4069.
Wesche-Soldato, D. E., et al. (2005) Blood 106: 2295-2301.
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