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Product Details
| Alternate Name | Ac-LEVD-AFC, Caspase-4 Substrate, Fluorogenic |
|---|---|
| Peptide Sequence | Ac-Leu-Glu-Val-Asp-AFC |
| Appearance | Liquid |
| Formulation | DMSO ( 1 mM) |
| Molecular Formula | C₃₂H₄₀F₃N₅O₁₁ |
| Molecular Weight | 727.5 |
| Purity | >99% by HPLC |
| SMILES | CC(C)CC(C(=O)NC(CCC(=O)O)C(=O)NC(C(C)C)C(=O)NC(CC(=O)O)C(=O)NC1=CC2=C(C=C1)C(=CC(=O)O2)C(F)(F)F)NC(=O)C |
| InChi | InChI=1S/C32H40F3N5O11/c1-14(2)10-21(36-16(5)41)30(49)38-20(8-9-24(42)43)28(47)40-27(15(3)4)31(50)39-22(13-25(44)45)29(48)37-17-6-7-18-19(32(33,34)35)12-26(46)51-23(18)11-17/h6-7,11-12,14-15,20-22,27H,8-10,13H2,1-5H3,(H,36,41)(H,37,48)(H,38,49)(H,39,50)(H,40,47)(H,42,43)(H,44,45) |
| InChi Key | FETZKXHXYOHAAN-UHFFFAOYSA-N |
| PubChem CID | 44135103 |
| MDL Number | MFCD01862605 |
| Handling | Protect from light and moisture |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
Ready-to-use fluorometric substrate for caspase-4 and related caspases that recognize the amino acid sequence LEVD. Caspase-4 and related caspase activity can be quantified by fluorescent detection of free AFC after cleavage from the peptide substrate LEVD-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized using a hand-held long-UV lamp.
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