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Caspase-4 Substrate LEVD-AFC

A caspase-4 substrate
Catalog #: 1109
SKU-Size Size Price Qty
1109-200 200 assays
1109-1000 1000 assays
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Product Details

Alternate Name Ac-LEVD-AFC, Caspase-4 Substrate, Fluorogenic
Peptide Sequence Ac-Leu-Glu-Val-Asp-AFC
Appearance Liquid
Formulation DMSO ( 1 mM)
Molecular Formula C₃₂H₄₀F₃N₅O₁₁
Molecular Weight 727.5
Purity >99% by HPLC
InChi InChI=1S/C32H40F3N5O11/c1-14(2)10-21(36-16(5)41)30(49)38-20(8-9-24(42)43)28(47)40-27(15(3)4)31(50)39-22(13-25(44)45)29(48)37-17-6-7-18-19(32(33,34)35)12-26(46)51-23(18)11-17/h6-7,11-12,14-15,20-22,27H,8-10,13H2,1-5H3,(H,36,41)(H,37,48)(H,38,49)(H,39,50)(H,40,47)(H,42,43)(H,44,45)
PubChem CID 44135103
MDL Number MFCD01862605
Handling Protect from light and moisture
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Ready-to-use fluorometric substrate for caspase-4 and related caspases that recognize the amino acid sequence LEVD. Caspase-4 and related caspase activity can be quantified by fluorescent detection of free AFC after cleavage from the peptide substrate LEVD-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized using a hand-held long-UV lamp.

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