Caspase-3 Substrate DEVD-AFC

Caspase-3 substrate

1007 is available from Abcam as ab285386.
Catalog #: 1007 | abID: ab285386

Product Details

abID ab285386
Alternate Name Ac-DEVD-AFC, Caspase-3 Substrate Fluorogenic
Peptide Sequence Ac-Asp-Glu-Val-Asp-AFC
Appearance Liquid
Formulation In DMSO (1 mM)
CAS # 1762-34-1
Molecular Formula C₃₀H₃₃F₃N₅O₁₃
Molecular Weight 729
Purity >98% by HPLC
Solubility DMSO
InChi InChI=1S/C30H34F3N5O13/c1-12(2)25(38-26(47)17(6-7-21(40)41)36-28(49)18(10-22(42)43)34-13(3)39)29(50)37-19(11-23(44)45)27(48)35-14-4-5-15-16(30(31,32)33)9-24(46)51-20(15)8-14/h4-5,8-9,12,17-19,25H,6-7,10-11H2,1-3H3,(H,34,39)(H,35,48)(H,36,49)(H,37,50)(H,38,47)(H,40,41)(H,42,43)(H,44,45)/t17-,18-,19-,25-/m0/s1
PubChem CID 644234
MDL Number MFCD01310970
Handling Potect from light and moisture
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Ready-to-use fluorometric substrate for caspase-3/CPP32 (Km = 9.7 µM) and related caspases that recognize the amino acid sequence DEVD. The sequence DEVD is based on caspase-3 cleavage site in poly (ADP-ribose) polymerase (PARP). CPP32 and related caspase activity can be quantified by fluorescent detection of free AFC after cleaved from the peptide substrate DEVD-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or a multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized using a hand-held long-UV lamp.

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