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Caspase-3 Substrate DEVD-AFC

based on 23 citations in multiple journalsCaspase-3 Substrate DEVD-AFC234.7 5
Caspase-3 substrate
Catalog #: 1007
SKU-Size Size Price Qty
1007-200 200 assays
$270.00
1007-1000 1000 assays
$570.00
More Sizes Get Quote

Product Details

Alternate Name Ac-DEVD-AFC, Caspase-3 Substrate Fluorogenic
Peptide Sequence Ac-Asp-Glu-Val-Asp-AFC
Appearance Liquid
Formulation In DMSO (1 mM)
CAS # 1762-34-1
Molecular Formula C₃₀H₃₃F₃N₅O₁₃
Molecular Weight 729
Purity >98% by HPLC
Solubility DMSO
SMILES CC(C)C(C(=O)NC(CC(=O)O)C(=O)NC1=CC2=C(C=C1)C(=CC(=O)O2)C(F)(F)F)NC(=O)C(CCC(=O)O)NC(=O)C(CC(=O)O)NC(=O)C
InChi InChI=1S/C30H34F3N5O13/c1-12(2)25(38-26(47)17(6-7-21(40)41)36-28(49)18(10-22(42)43)34-13(3)39)29(50)37-19(11-23(44)45)27(48)35-14-4-5-15-16(30(31,32)33)9-24(46)51-20(15)8-14/h4-5,8-9,12,17-19,25H,6-7,10-11H2,1-3H3,(H,34,39)(H,35,48)(H,36,49)(H,37,50)(H,38,47)(H,40,41)(H,42,43)(H,44,45)/t17-,18-,19-,25-/m0/s1
InChi Key GZDRODOYEFEHGG-NUDCOPPTSA-N
PubChem CID 644234
MDL Number MFCD01310970
Handling Potect from light and moisture
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Ready-to-use fluorometric substrate for caspase-3/CPP32 (Km = 9.7 µM) and related caspases that recognize the amino acid sequence DEVD. The sequence DEVD is based on caspase-3 cleavage site in poly (ADP-ribose) polymerase (PARP). CPP32 and related caspase activity can be quantified by fluorescent detection of free AFC after cleaved from the peptide substrate DEVD-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or a multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized using a hand-held long-UV lamp.


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Sin et al., Acute Treatment of Resveratrol Alleviates Doxorubicin-Induced Myotoxicity in Aged Skeletal Muscle Through SIRT1-Dependent Mechanisms.  J Gerontol A Biol Sci Med Sci, Oct 2015; 10.1093/gerona/glv175.
Pei et al., Desacyl ghrelin prevents doxorubicin-induced myocardial fibrosis and apoptosis via the GHSR-independent pathway. Am J Physiol Endocrinol Metab, Feb 2014; 306: E311 - E323.
Chen et al., Infection of Pericytes In Vitro by Japanese Encephalitis Virus Disrupts the Integrity of the Endothelial Barrier. J. Virol., Jan 2014; 88: 1150 - 1161.
Ren, K. et al. Tumor-suppressing Function of Caspase-2 Requires Catalytic Site Cys-320 and Site Ser-139 in Mice, J. Biol. Chem., Apr 2012; 287: 14792 - 14802.
Rui,T. et al.,Reduction in IL-33 expression exaggerates ischaemia/reperfusion-induced myocardial injury in mice with diabetes mellitus, Cardiovasc Res, Feb 2012; 10.1093/cvr/cvs015.
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