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Product Details
| Alternate Name | Ac-DEVD-AFC, Caspase-3 Substrate Fluorogenic |
|---|---|
| Peptide Sequence | Ac-Asp-Glu-Val-Asp-AFC |
| Appearance | Liquid |
| Formulation | In DMSO (1 mM) |
| CAS # | 1762-34-1 |
| Molecular Formula | C₃₀H₃₃F₃N₅O₁₃ |
| Molecular Weight | 729 |
| Purity | >98% by HPLC |
| Solubility | DMSO |
| SMILES | CC(C)C(C(=O)NC(CC(=O)O)C(=O)NC1=CC2=C(C=C1)C(=CC(=O)O2)C(F)(F)F)NC(=O)C(CCC(=O)O)NC(=O)C(CC(=O)O)NC(=O)C |
| InChi | InChI=1S/C30H34F3N5O13/c1-12(2)25(38-26(47)17(6-7-21(40)41)36-28(49)18(10-22(42)43)34-13(3)39)29(50)37-19(11-23(44)45)27(48)35-14-4-5-15-16(30(31,32)33)9-24(46)51-20(15)8-14/h4-5,8-9,12,17-19,25H,6-7,10-11H2,1-3H3,(H,34,39)(H,35,48)(H,36,49)(H,37,50)(H,38,47)(H,40,41)(H,42,43)(H,44,45)/t17-,18-,19-,25-/m0/s1 |
| InChi Key | GZDRODOYEFEHGG-NUDCOPPTSA-N |
| PubChem CID | 644234 |
| MDL Number | MFCD01310970 |
| Handling | Potect from light and moisture |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
Ready-to-use fluorometric substrate for caspase-3/CPP32 (Km = 9.7 µM) and related caspases that recognize the amino acid sequence DEVD. The sequence DEVD is based on caspase-3 cleavage site in poly (ADP-ribose) polymerase (PARP). CPP32 and related caspase activity can be quantified by fluorescent detection of free AFC after cleaved from the peptide substrate DEVD-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or a multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized using a hand-held long-UV lamp.
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