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Caspase-3, rat recombinant

A cysteine-aspartic acid protease involved in apoptosis
Catalog #: 1283
SKU-Size Size Price Qty
1283-100 100 units
$285.00
1283-25 25 units
$145.00
More Sizes Get Quote

Product Details

Alternate Name Caspase-3, Short name=CASP-3, Apopain, Cysteine protease CPP32, Short name=CPP-32, IRP, LICE, Protein Yama, SREBP cleavage activity 1, Short name=SCA-1
Source E. coli
Appearance Lyophilized powder
Physical Form Description Lyophilized powder
Molecular Weight large (17 kD) and small (11 kD) subunits
Reconstitution Instructions Reconstitute to 1 unit per µl in PBS containing 15% glycerol.
Handling Centrifuge the vial prior to opening.
Storage Conditions -70°C
Shipping Conditions Dry Ice
USAGE For Research Use Only! Not to be used in humans

Details

Caspase-3 (also know as CPP32, Yama and apopain) is a major member of the caspase-family of cysteine proteases. Caspase-3 exists in cells as an inactive 32 kDa proenzyme. During apoptosis procaspase-3 is processed at aspartate residues by self-proteolysis and/or cleavage by upstream caspases, such as caspase-6 (Mch2), caspase-8 (Flice) and grazyme B. The processed form of caspase-3 consists of large (17 kD) and small (11 kD) subunits which associate to form the active enzyme. The active caspase-3 has been shown involving in the proteolysis of several important molecules, such as poly (ADP-ribose) polymerase (PARP), the sterol regulatory element binding proteins (SREBPs), focal adhesion kinase (FAK), and others. The recombinant active human caspase-3 expressed in E. coli spontaneously undergoes autoprocessing to yield subunits characteristic of the native enzyme. The active caspase-3 preferentially cleaves caspase-3 substrates (e.g., DEVD-AFC or DEVD-pNA) and is routinely tested at BioVision for its ability to enzymatically cleave these two substrates Ac-DEVD-pNA (Cat. #1008-200) or Ac-DEVD-AFC (1007-200).


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