Caspase-3 Activity Assay Kit II (Fluorometric)

A sensitive assay kit for the quantification of caspase 3 activity in mammalian cell culture. Standard and Positive Control included.
Catalog #: K533 | abID: ab252897

Product Details

abID ab252897
Cat # +Size K533-100
Size 100 assays
Detection Method Fluorometric (Ex/Em =400/505 nm)
Species Reactivity Mammalian
Applications Comparison between apoptotic sample an uninduced control; allows determination of the fold increase in caspase-3/CPP32 activity
Features & Benefits • Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.
• Fluorometric (400/505 nm)
• Simple, 1-Step Procedure; takes 1-2 hours to complete,Complete kit. Includes positive control and AFC as standard
Kit Components • Staurosporine
• Ac-DEVD-AFC
• Caspase Cell Lysis Buffer
• AFC Standard (1 mM)
• DTT (1 M)
• Caspase 2X Reaction Buffer
• Caspase-3 (Positive Control)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Caspase-3 (3.4.22.56) is a cysteine protease that cleaves the C-terminal of an Aspartate residue at the P1 position. The cleavage event is a critical step in the downstream signaling and amplification of apoptosis, or programmed cell death. Apoptosis is a tightly regulated event that plays a central role in the development and homeostasis of multi-cellular organisms. BioVision’s Caspase-3 Fluorometric Activity Assay Kit II allows the detection and quantification of Caspase-3 Activity by using a synthetic substrate DEVD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin), which upon cleavage by Caspase-3, will emit a strong, stable fluorometric signal (Ex/Em= 400/505 nm). The assay is sensitive, fast and allows for the high-throughput quantification of Caspase-3 Activity in cell lysate or adherent cells and can be used to evaluate induction of apoptosis and/or evaluation of apoptosis inhibitors. As little as 11.25 mU of activity per well and less than 500 apoptotic cells can be detected


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Ping Zhou, Protective Effects of Total Saponins of Aralia elata (Miq.) on Endothelial Cell Injury Induced by TNF-α via Modulation of the PI3K/Akt and NF-κB Signalling Pathways. Int J Mol Sci, Dec 2018; 30577658.
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