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Product Details
| Alternate Name | Ac-VDVAD-pNA, Caspase-2 Substrate, Colorimetric, VDVAD-pNA |
|---|---|
| Peptide Sequence | Ac-Val-Asp-Val-Ala-Asp-pNA |
| Appearance | Liquid |
| Formulation | DMSO (4 mM) |
| Molecular Formula | C₂₉H₄₁N₇O₁₂ |
| Molecular Weight | 679.7 |
| Purity | >98% by HPLC |
| SMILES | CC(C)C(C(=O)NC(CC(=O)O)C(=O)NC(C(C)C)C(=O)NC(C)C(=O)NC(CC(=O)O)C(=O)NC1=CC=C(C=C1)[N+](=O)[O-])NC(=O)C |
| InChi | InChI=1S/C29H41N7O12/c1-13(2)23(31-16(6)37)29(46)34-20(12-22(40)41)27(44)35-24(14(3)4)28(45)30-15(5)25(42)33-19(11-21(38)39)26(43)32-17-7-9-18(10-8-17)36(47)48/h7-10,13-15,19-20,23-24H,11-12H2,1-6H3,(H,30,45)(H,31,37)(H,32,43)(H,33,42)(H,34,46)(H,35,44)(H,38,39)(H,40,41) |
| InChi Key | SIOKOOKURWWOID-UHFFFAOYSA-N |
| PubChem CID | 44135308 |
| Handling | Protect from light and moisture |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
Ready-to-use colorimetric substrate for caspase-2/Ich-1 and related caspases that recognize the amino acid sequence VDVAD. Caspase-2 and related caspase activity can be quantified by detection of free pNA after cleavage from the peptide substrate VDVAD-pNA at OD405 nm using a spectrophotometer or plate reader. The ready-to-use caspase substrate provides an economic alternative for researchers who perform large amount of caspase assays.
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Basu A et al (2008) Cancer Res. 68: 2795-3802.
Lee S-Y et al. (2007) Infect Immun. 75: 1291-1302.
Doostzadeh J., et al. (2000) J. Biol. Chem. 275:25336-25341.
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