Caspase-12 Substrate ATAD-AFC

A caspase-12 substrate
Catalog #: 1117 | abID:

Product Details

Alternate Name Ac-ATAD-AFC, Caspase-12 Substrate, Fluorogenic
Peptide Sequence Ac-Ala-Thr-Ala-Asp-AFC
Appearance Liquid
Formulation DMSO (4 mM)
Molecular Formula C₂₆H₃₀F₃N₅O₁₀
Molecular Weight 629.54
Purity >90% by HPLC
Handling Protect from light and moisture
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Ready-to-use fluorometric substrate for caspases that recognize the amino acid sequence ATAD. Caspase activity can be quantified by fluorescent detection of free AFC after cleaved from the peptide substrate ATAD-AFC at Ex/Em = 400/505 nm using a fluorometer or a fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized using a hand-held long-UV lamp. The ready-to-use caspase substrate provides an economic alternative for researchers who perform large amount of caspase assays. Cell Lysis Buffer (Cat.# 1067-100), 2X Reaction Buffer (Cat.# 1068-20), DTT (Cat.# 1201-1), and other reagents used for caspase activity assays are also available separately.

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Baumgartner H et al. (2007) Am. J. Physiol. Gastrointest. Liver Physiol. 10.1152/ajpgi.00103.2007.
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