Caspase-1 Substrate YVAD-AFC

A caspase-1 substrate
Catalog #: 1103 | abID:

Product Details

Alternate Name Ac-YVAD-AFC, Caspase-1 Substrate, Fluorogenic
Peptide Sequence Ac-Tyr-Val-Ala-Asp-AFC
Appearance Liquid
Formulation DMSO ( 1 mM)
Molecular Formula C₃₃H₃₆F₃N₅O₁₀
Molecular Weight 719.7
Purity >99% by HPLC
InChi InChI=1S/C33H36F3N5O10/c1-15(2)28(41-31(49)23(38-17(4)42)11-18-5-8-20(43)9-6-18)32(50)37-16(3)29(47)40-24(14-26(44)45)30(48)39-19-7-10-21-22(33(34,35)36)13-27(46)51-25(21)12-19/h5-10,12-13,15-16,23-24,28,43H,11,14H2,1-4H3,(H,37,50)(H,38,42)(H,39,48)(H,40,47)(H,41,49)(H,44,45)
PubChem CID 44135090
MDL Number MFCD01318881
Handling Protect from light and moisture
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Ready-to-use fluorometric substrate for caspase-1/ICE and related caspases that recognize the amino acid sequence YVAD. Caspase-1 and related caspase activity can be quantified by fluorescent detection of free AFC after cleaved from the peptide substrate YVAD-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized, using a hand-held long-UV lamp. The ready-to-use caspase substrate provides an economic alternative for researchers who perform large volume caspase assays. Cell Lysis Buffer (Cat. #1067-100, -400) and 2X Reaction Buffer (Cat. #1068-20, -80), and DTT used for caspase assays are also available separately.

Why buy BioVision Products?
Global Presence
Technical Support
BioVision aims to provide our customers innovative tools for accelerating drug discovery and biological research. BioVision offers >8,000 products including the most comprehensive array of assay kits for key targets in Metabolic pathways.
BioVision is committed to providing the highest quality products at a competitive price.
We have a broad network of global distributors who are ready to address your research needs and ensure fast delivery.
Our highly trained Technical Support team provides comprehensive product support and is dedicated to resolving your issues quickly and efficiently.
Ray, D. M., et al. (2005) J. Immunol. 174: 4060-4069.
Bai, S., et al. (2004) Am. Cancer Ther. 3:613-620.
Coletti, D., et al. (2002) EMBO J. 21:631-642.
For more citations of this product click here