Product Details
| Alternate Name | Ac-YVAD-AFC, Caspase-1 Substrate, Fluorogenic |
|---|---|
| Peptide Sequence | Ac-Tyr-Val-Ala-Asp-AFC |
| Appearance | Liquid |
| Formulation | DMSO ( 1 mM) |
| Molecular Formula | C₃₃H₃₆F₃N₅O₁₀ |
| Molecular Weight | 719.7 |
| Purity | >99% by HPLC |
| SMILES | CC(C)C(C(=O)NC(C)C(=O)NC(CC(=O)O)C(=O)NC1=CC2=C(C=C1)C(=CC(=O)O2)C(F)(F)F)NC(=O)C(CC3=CC=C(C=C3)O)NC(=O)C |
| InChi | InChI=1S/C33H36F3N5O10/c1-15(2)28(41-31(49)23(38-17(4)42)11-18-5-8-20(43)9-6-18)32(50)37-16(3)29(47)40-24(14-26(44)45)30(48)39-19-7-10-21-22(33(34,35)36)13-27(46)51-25(21)12-19/h5-10,12-13,15-16,23-24,28,43H,11,14H2,1-4H3,(H,37,50)(H,38,42)(H,39,48)(H,40,47)(H,41,49)(H,44,45) |
| InChi Key | GWKJISJKNKIJNP-UHFFFAOYSA-N |
| PubChem CID | 44135090 |
| MDL Number | MFCD01318881 |
| Handling | Protect from light and moisture |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
Ready-to-use fluorometric substrate for caspase-1/ICE and related caspases that recognize the amino acid sequence YVAD. Caspase-1 and related caspase activity can be quantified by fluorescent detection of free AFC after cleaved from the peptide substrate YVAD-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized, using a hand-held long-UV lamp. The ready-to-use caspase substrate provides an economic alternative for researchers who perform large volume caspase assays. Cell Lysis Buffer (Cat. #1067-100, -400) and 2X Reaction Buffer (Cat. #1068-20, -80), and DTT used for caspase assays are also available separately.
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Ray, D. M., et al. (2005) J. Immunol. 174: 4060-4069.
Bai, S., et al. (2004) Am. Cancer Ther. 3:613-620.
Coletti, D., et al. (2002) EMBO J. 21:631-642.
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