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Anti-Cas9 Antibody (7A9)

Monoclonal antibody to detect Cas9.
Catalog #: A1069

In stock

$475.00

Product Details

Cat # +Size A1069-50
Size 50 µg
Antibody Target Cas9
Alternate Name CRISPR-associated endonuclease Cas9/Csn1, CRISPR associated protein 9, SpyCas9
Host Mouse
Antibody Type Monoclonal
Isotype Mouse IgG1κ
Immunogen N-terminal fragment of Streptococcus pyogenes Cas9.
Accession # Q99ZW2
Gene ID 901176
Appearance Colorless liquid
Formulation PBS containing 0.02% sodium azide.
Purification Affinity purified using protein G
Application IF, IP, WB
Positive Control IF, IP, WB
Application & Usage Immunofluorescence: 1:500-1:2,000; Immunoprecipitation: 1:20; Western Blot: 1:1,000
Handling The antibody solution should be gently mixed before use.
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Cas9 (CRISPR associated protein 9) is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspersed Palindromic Repeats) adaptive immunity system in Streptococcus pyogenes and other bacteria. Cas9 is used to interrogate and cleave foreign DNA, such as invading bacteriophage DNA or plasmid DNA. This is accomplished by unwinding foreign DNA and checking for if it is complementary to the 20 base pair spacer region of the guide RNA. If the DNA substrate is complementary to the guide RNA, Cas9 cleaves the invading DNA.


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