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Cardiolipin Assay Kit (Fluorometric)

The easiest kit in the market to measure Cardiolipin in samples
Catalog #: K944

Product Details

Cat # +Size K944-100
Size 100 assays
Detection Method Fluorescence (Ex/Em 340/480 nm)
Species Reactivity Mammalian
Applications This assay provides a convenient and non-ELISA alternative to detect cardiolipin amount in biological samples.
Features & Benefits Simple, fast, does not require lenghty incubation times
Kit Components • CL Assay Buffer
• Probe
• Cardiolipin (5 mM)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Cardiolipin (CL) is a very important phospholipid present in the inner mitochondrial membrane and constitutes about 20% of its total lipid composition. It has a dimeric structure comprised of two phosphatidic acid residues linked by a glycerol bridge. It is essential for several mitochondrial functions such as maintaining activity of the electron transport chain complexes, and other mitochondrial processes including biogenesis, fission, fusion and protein transport. It is involved in apoptosis where it helps alter the mitochondrial membrane structure and aids in the release of cytochrome c. Condition such as diabetes and heart failure are linked to changes in levels of cardiolipin and it is also depleted during aging. Exposure to toxicants like cigarette smoke and organophosphates may cause an alteration in cardiolipin levels and composition, thus adversely affecting health. BioVision’s Cardiolipin Assay is a fluorometric assay that makes use of a proprietary probe that fluoresces on association with cardiolipin but not with any other lipids such as phosphatidylcholine and sphingomyelin, making it highly specific. Fluorescence can be recorded at Ex/Em 340/480 nm. The kit includes purified cardiolipin as standard and can detect as low as 0.2 nmol of cardiolipin

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Does the kit detect the oxidized or the unoxidized form of Cardiolipin?
The assay will not differentiate between the oxidized and unoxidized Cardiolipin. The CL probe binds to both types of Cardiolipin and fluorescence is generated as result of structural changes to the probe on association with Cardiolipin. The probe does not differentiate between the oxidized and unoxidized Cardiolipin.