Carboxylesterase Activity Assay Kit (Fluorometric) (ab273314)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Fluorescent
- Platform: Microplate
- Sample type: Cell Lysate, Tissue Lysate
- Sensitivity: 6.6 µU
Overview
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Product name
Carboxylesterase Activity Assay Kit (Fluorometric)
See all Carboxylesterase 1 kits -
Detection method
Fluorescent -
Sample type
Cell Lysate, Tissue Lysate -
Assay type
Enzyme activity (quantitative) -
Sensitivity
6.6 µU -
Assay duration
Multiple steps standard assay -
Product overview
Carboxylesterase Activity Assay Kit (Fluorometric) (ab273314) is a simple, rapid, plate-based fluorometric assay for measuring Carboxylesterase activity in biological samples. The kit uses the proprietary substrate for the quantification of Carboxylesterase (CE) activity in samples.
The assay can detect as low as 6.6 µU of Carboxylesterase activity.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K2014 Carboxylesterase Activity Assay Kit (Fluorometric). K2014-100 is the same size as the 100 test size of ab273314.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests CES Assay Buffer 1 x 13ml CES1 Positive Control 1 vial CES1 Fluorescence Standard 1 x 50µl CES1 Substrate 1 x 50µl -
Research areas
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Alternative names
- ACAT
- Acyl coenzyme A:cholesterol acyltransferase
- Brain carboxylesterase hBr1
see all
Images
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Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Carboxylesterase Standard Curve (0-500 pmoles/well).
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Example data.
Carboxylesterase activity in human liver microsomes (HLM) (16 mg/ml), and HepG2 lysate (3.21 mg/ml).
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Example data.
Carboxylesterase activity in HepG2 lysate, human liver microsomes and Positive Control.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab273314 has not yet been referenced specifically in any publications.