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Carboxylesterase 1D, mouse recombinant

Protein involved in the metabolism of xenobiotics and of natural substrates
Catalog #: P1094
SKU-Size Size Price Qty
P1094-2 2 μg
$95.00
P1094-10 10 μg
$415.00
More Sizes Get Quote

Product Details

Alternate Name Ces1d, Carboxylesterase 3, FAEE synthase, TGH, Ces3
Gene Symbol Ces1d
Gene ID 104158
Accession # Q8VCT4
Source Insect cell (Baculovirus)
Appearance Liquid
Physical Form Description In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
Molecular Weight 60.9 kDa
Purity by SDS-PAGE ≥90%
Endotoxin Level < 1 EU/μg
Biological Activity > 80,000 pmol/min/ug and is defined as the amount of enzyme that hydrolyze 1pmole of p-nitrophenyl acetate to pnitrophenol per minute at pH 7.5 at 37C.
Amino Acid Sequence YPSSPPVVNT VKGKVLGKYV NLEGFTQPVA VFLGVPFAKP PLGSLRFAPP QPAEPWSFVK NTTSYPPMCS QDAVGGQVLS ELFTNRKENI PLQFSEDCLY LNIYTPADLT KNSRLPVMVW IHGGGLVVGG ASTYDGLALS AHENVVVVTI QYRLGIWGFF STGDEHSRGN WGHLDQVAAL RWVQDNIANF GGNPGSVTIF GESAGGFSVS VLVLSPLAKN LFHRAISESG VS
Handling Centrifuge the vial prior to opening.
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not to be used in humans

Details

Ces1d, also known as carboxylesterase 1D, is a member of a large family of carboxylesterases that are responsible for the hydrolysis of ester and amide bonds. It is the principle lipase of white adipose tissue fat cake extracts. Partially purified white adipose tissue Ces1d had lipase activity as well as lesser but detectable neutral cholesteryl ester hydrolase activity. The protein shows low catalytic efficiency for hydrolysis of CPT-11, a prodrugs for camptothecin used in cancer therapeutics. Recombinant mouse Ces1d, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.


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