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Carbonic Anhydrase 3, human recombinant

Catalyzes the reversible hydration of carbon dioxide
Catalog #: 7833

In stock

SKU-Size Size Price Qty
7833-10 10 μg
$180.00
7833-50 50 μg
$560.00
More Sizes Get Quote

Product Details

Alternate Name CA3, Carbonic anhydrase 3
Gene Symbol CA3
Gene ID 761
Accession # P07451
Source E. coli.
Appearance Lyophilized
Physical Form Description Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.
Molecular Weight This protein is fused with polyhistidine tag at the C-terminus, and has a calculated MW of 30.4 kDa. The predicted N-terminus is Met 1. DTT-reduced Protein migrates as 28-30 kDa in SDS-PAGE.
Purity by SDS-PAGE ≥95%
Endotoxin Level <1 EU/μg by LAL method
Biological Activity Measured by its esterase activity. The specific activity is >1 pmol/min/µg.
Reconstitution Instructions Centrifuge the vial prior to opening. Reconstitute in PBS, pH 7.4. Do not vortex.
Handling Centrifuge the vial prior to opening.
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not to be used in humans

Details

Carbonic anhydrase 3 (CA3) is also known as Carbonate dehydratase III, Carbonic anhydrase III, which belongs to the alpha-carbonic anhydrase family. These carbonic anhydrases are a class of metalloenzymes that catalyze the reversible hydration of carbon dioxide and are differentially expressed in a number of cell types. The catalytic activity of carbonic anhydrase 3 is activated by proton donors such as imidazole and the dipeptide histidylhistidine and is inhibited by coumarins and sulfonamide derivatives such as acetazolamide.


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