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Carbonic anhydrase-1, human recombinant

Catalyzes the reversible hydration of carbon dioxide in various biological processes.
Catalog #: P1048
SKU-Size Size Price Qty
P1048-10 10 μg
$105.00
P1048-50 50 μg
$305.00
More Sizes Get Quote

Product Details

Alternate Name CA1, CA-I, CAB
Gene Symbol CA1
Gene ID 759
Accession # P00915
Source E. coli
Appearance Liquid
Physical Form Description In 20 mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 10% glycerol
Molecular Weight 31 kDa
Purity by SDS-PAGE > 95%
Biological Activity Specific activity is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 8 at 37C.
Amino Acid Sequence MGSSHHHHHH SSGLVPRGSH MASPDWGYDD KNGPEQWSKL YPIANGNNQS PVDIKTSETK HDTSLKPISV SYNPATAKEI INVGHSFHVN FEDNDNRSVL KGGPFSDSYR LFQFHFHWGS TNEHGSEHTV DGVKYSAELH VAHWNSAKYS SLAEAASKAD GLAVIGVLMK VGEANPKLQK VLDALQAIKT KGKRAPFTNF DPSTLLPSSL DFWTYPGSLT HPPLYESVTW II
Handling Centrifuge the vial prior to opening.
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not to be used in humans

Details

Carbonic anhydrase (CA) is an enzyme that catalyses rapid conversion of carbon dioxide to bicarbonate and protons (CO2 + H2O ↔ HCO3− + H+). Most carbonic anhydrases contain a zinc ion in their active site and the primary function of this enzyme is known to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide of tissues. Carbonic anhydrases have been found in all kingdoms of life. Recombinantcarbonic anhydrase fused to His-tag, was expressed in E.coli and purified by conventional chromatography techniques. Specific activity is >450 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C.


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