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Calprotectin (Human) ELISA Kit

A Sandwich ELISA kit for the quantitative measurement of Calprotectin in human serum, plasma, tissue lysates, and other biological fluids
Catalog #: E4889
$695.00

Product Details

Cat # +Size E4889-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Human
Applications This Sandwich ELISA kit is designed to quantitatively measure amount of Calprotectin in human serum, plasma, tissue lysates, and other biological fluids
Features & Benefits • Assay Precision: Intra-Assay CV < 8% and Inter-Assay CV < 10%
• Recovery range: 85 - 105% for normal human serum and plasma samples
• This Sandwich ELISA is highly sensitive and highly specific for the detection of Calprotectin in human samples. There is no significant cross-reactivity or interference between Calprotectin and analogues
• Detection range: 15.625 - 1000 ng/ml
• Sensitivity: 9.375 ng/ml
Kit Components • Micro ELISA plate
• Standard (Lyophilized) (1000 ng)
• Sample/Standard Dilution Buffer
• Biotin-labeled Antibody
• Antibody Dilution Buffer
• HRP-Streptavidin Conjugate (SABC)
• SABC Dilution Buffer
• TMB Substrate Solution
• Stop Solution
• Wash Buffer (25X)
• Plate Sealers
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Calprotectin is a calcium and zinc-binding protein that belongs to the family of S100 proteins. It is a 24 kDa dimer that is formed by monomers S100A8 and S100A9. It is predominantly present in neutrophils but less in monocytes and macrophages. Calprotectin contributes to approximately 60% of the cytosolic proteins in neutrophils. It has bactericidal and fungicidal properties and increases 5-40 folds during infection and inflammation. The concentration of Calprotectin in stool samples is 6 times higher than the blood plasma. Therefore it serves as a reliable non-invasive marker for inflammatory bowel diseases such as ulcerative colitis and Crohn's disease. BioVision’s Calprotectin (Human) ELISA kit is based on the Sandwich ELISA principle to quantitatively measure the amount of Calprotectin in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. The HRP-Streptavidin is added and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the amount of Calprotectin in the sample or standard.


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