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Product Details
| Highlights | FORMULATION: Supplied as 50% aqueous slurry in 20 % Ethanol. LIGAND DENSITY: ~ 1.5 mg Calmodulin protein per ml of drained resin/beads. PREPARATION: Calmodulin-Sepharose Beads are prepared by covalently coupling recombinant human calmodulin (Cat # 7838) to 6% cross linked Sepharose beads. APPLICATIONS: • Single-step purification of native calmodulin-binding proteins. • Tandem affinity purification (TAP) of protein complexes. • Purification of CBP tagged proteins. • Purification of calmodulin-regulated proteins from all eukaryotic cells. |
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| Storage Conditions | 4°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
Calmodulin is a highly conserved regulatory eukaryotic protein involved in various cellular processes such as glycogen metabolism, cytoskeletal control, neurotransmission, phosphate activity and control of NAD/NADP. The diverse functions of calmodulin rely on its ability to interact with, and regulate the activities of large number of its target proteins/enzymes. Calmodulin binds proteins usually through its hydrophobic sites, which are exposed due to conformational changes in the presence of Ca2+. However, some target proteins interact with Calmodulin in a calcium-independent manner. Calmodulin-Sepharose beads allow for specific enrichment of known or unknown calmodulin-binding proteins from biological samples. In addition, Calmodulin-Sepharose beads provide a convenient tool for affinity purification of calmodulin binding peptide (CBP) tagged recombinant proteins. Elution of CBP tagged proteins could be achieved by simply adding chelation ligand / removing Ca2+.
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Can it also work to pull down mouse and rat calmodulin interacting proteins?
Protein blast search against the human Calmodulin sequence shows a 99% match with mouse (Mus musculus) calmodulin and a 100% match with rat (Rattus norvegicus) calmodulin. Thus the beads should be able to pulldown mouse and rat calmodulin interacting proteins.
Eli J. Rogers, An IQSEC2 Mutation Associated With Intellectual Disability and Autism Results in Decreased Surface AMPA Receptors. Front Mol Neurosci, Feb 2019; 30842726.
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