Calcein AM

A fluorescent dye
Catalog #: 1755 | abID:

Product Details

Alternate Name Glycine,N,N'-[[3',6'-bis(acetyloxy)-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-2',7'-diyl]bis(methylene)]bis[N-[2-[(acetyloxy)methoxy]-2-oxoethyl]-,bis[(acetyloxy)methyl] ester; Calcein acetoxymethyl ester
Appearance Off-white solid
CAS # 148504-34-1
Molecular Formula C₄₆H₄₆N₂O₂₃
Molecular Weight 994.86
Purity ≥95% by HPLC
Solubility DMSO (5mg/ml)
InChi InChI=1S/C46H46N2O23/c1-25(49)60-21-64-41(55)17-47(18-42(56)65-22-61-26(2)50)15-31-11-35-39(13-37(31)68-29(5)53)70-40-14-38(69-30(6)54)32(12-36(40)46(35)34-10-8-7-9-33(34)45(59)71-46)16-48(19-43(57)66-23-62-27(3)51)20-44(58)67-24-63-28(4)52/h7-14H,15-24H2,1-6H3
PubChem CID 390986
MDL Number MFCD05861516
Handling Protect from air and light
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


A cell-permeable, non-fluorescent acetoxymethyl ester derivative of Calcein that gets hydrolyzed inside the cell by esterases. The loss of the acetoxymethyl group also enables calcein to readily bind intracellular calcium resulting in a strong yellowish-green fluorescence. It is the best probe for cell viablilty and cell adhesion due to its greater retention in cells as well and its pH insensitivity in physiological range.

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