Product Details
| Alternate Name | Glycine,N,N'-[[3',6'-bis(acetyloxy)-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-2',7'-diyl]bis(methylene)]bis[N-[2-[(acetyloxy)methoxy]-2-oxoethyl]-,bis[(acetyloxy)methyl] ester; Calcein acetoxymethyl ester |
|---|---|
| Appearance | Off-white solid |
| CAS # | 148504-34-1 |
| Molecular Formula | C₄₆H₄₆N₂O₂₃ |
| Molecular Weight | 994.86 |
| Purity | ≥95% by HPLC |
| Solubility | DMSO (5mg/ml) |
| SMILES | CC(=O)OCOC(=O)CN(CC1=C(C=C2C(=C1)C3(C4=CC=CC=C4C(=O)O3)C5=CC(=C(C=C5O2)OC(=O)C)CN(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)OC(=O)C)CC(=O)OCOC(=O)C |
| InChi | InChI=1S/C46H46N2O23/c1-25(49)60-21-64-41(55)17-47(18-42(56)65-22-61-26(2)50)15-31-11-35-39(13-37(31)68-29(5)53)70-40-14-38(69-30(6)54)32(12-36(40)46(35)34-10-8-7-9-33(34)45(59)71-46)16-48(19-43(57)66-23-62-27(3)51)20-44(58)67-24-63-28(4)52/h7-14H,15-24H2,1-6H3 |
| InChi Key | BQRGNLJZBFXNCZ-UHFFFAOYSA-N |
| PubChem CID | 390986 |
| MDL Number | MFCD05861516 |
| Handling | Protect from air and light |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
A cell-permeable, non-fluorescent acetoxymethyl ester derivative of Calcein that gets hydrolyzed inside the cell by esterases. The loss of the acetoxymethyl group also enables calcein to readily bind intracellular calcium resulting in a strong yellowish-green fluorescence. It is the best probe for cell viablilty and cell adhesion due to its greater retention in cells as well and its pH insensitivity in physiological range.
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