Caffeine ELISA Kit

A competitive ELISA kit for the quantitative measurement of Caffeine in serum, urine and saliva.

WARNING: This product can expose you to chemicals including Sulfuric acid and TMB, which are known to the State of California to cause cancer. For more information go to
Catalog #: E4558 | abID: ab285229

Product Details

abID ab285229
Cat # +Size E4558-100
Size 100 assays
Kit Summary • Detection method- Absorbance (450 nm)
• Species reactivity- Universal
• Application- quantitative measurement of Caffeine in serum, urine and saliva
Detection Method Absorbance (450 nm)
Species Reactivity Universal
Applications Quantitative measurement of Caffeine in serum, urine and saliva
Features & Benefits • This ELISA kit is used for in vitro quantitative determination of caffeine.
• Detection Range: 0.33 – 27 ng/ml
• Sensitivity: 0.3 ng/ml
Kit Components • ELISA Microplate
• Standard
• HRP-conjugate
• Antibody
• TMB Substrate
• Stop Solution
• Sample Diluent
• Wash Buffer (10X)
• Serum Solution
• Standard Buffer
• Plate Sealers
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Caffeine (1,3,7-trimethylxanthine) is a methylxanthine alkaloid which acts as a stimulant for central nervous system that can be used to increase blood pressure and reduce fatigue. Caffeine is naturally present in leaves, seeds, nuts and a number of plants. It is also known to have anti-inflammatory effect by inhibiting adenosine monophosphate phosphodiesterase. Nowadays, many common beverages including coffee, tea, sodas and energy drinks contain different levels of caffeine to relieve drowsiness and improve performance. At normal dose, caffeine generally improves reaction time, wakefulness, concentration and motor coordination. However, caffeine overdose in the body can lead to nervousness, bone loss, headache, anxiety, insomnia and even death. BioVision’s Caffeine ELISA Kit is a competitive-based ELISA that can quantify as low as 0.3 ng/ml caffeine in urine, saliva and serum samples within 90 minutes.

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Can we use coffee powder as a sample with this kit? Do you have any data?
The kit should be able to detect caffeine from coffee powder. We haven’t tested the coffee powder but it should be easy to test as it is highly concentrated with caffeine. There is 57 mg of caffeine in 1.8 g of coffee (3.2%). So 1 mg of coffee powder in 1 ml of water is 32 µg/ml of caffeine. We do not anticipate the sample color to be a problem as the dilution factor is at least >500. However, the customer needs to use high speed to centrifuge the coffee sample or use 10 kDa filter to remove the undissolved powder or debris.