C5a, human recombinant

A potent chemotactic factor for human peripheral blood neutrophils and monocytes
Catalog #: 4995B | abID: ab61918

Product Details

abID ab61918
Alternate Name C5a anaphylatoxin chemotactic receptor, C5a anaphylatoxin chemotactic receptor (C5a-R), (C5aR,) (CD antigen CD88), C5AR1, C5AR, C5R1
Source E. coli
Appearance Lyophilized protein
Physical Form Description Lyophilized with no additives
Molecular Weight (74 amino acids)
Purity by SDS-PAGE ≥95%
Endotoxin Level <0.1 ng/μg
Biological Activity Human recombinant C5a is as active as that of serum derived C5a based on enzyme release assay of myeloperoxidase. The current lot of ED₅₀ = 1.36 nmolar.
Reconstitution Instructions Reconstitute to a concentration of 1.0 mg/ml in ice-cold H₂O containing 2.5 mg/ml BSA. The solution should be aliquoted and immediately stored at -70°C for future use. Frozen/thaw will result in some loss of activity.
Handling Centrifuge the vial prior to opening.
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not to be used in humans

Details

Human complement C5a is a 74-amino acid glycopeptide generated during complement activation from the α-chain of complement C5. C5a is a potent chemotactic factor for human peripheral blood neutrophils and monocytes, and is believed to play an important role in a number of inflammatory conditions. The 74 amino acid, human C5a protein expressed in E. coli has an activity similar to that of serum derived C5a based on enzyme assay of myeloperoxidase release.


Why buy BioVision Products?
Innovation
Affordability
Global Presence
Technical Support
BioVision aims to provide our customers innovative tools for accelerating drug discovery and biological research. BioVision offers >8,000 products including the most comprehensive array of assay kits for key targets in Metabolic pathways.
BioVision is committed to providing the highest quality products at a competitive price.
We have a broad network of global distributors who are ready to address your research needs and ensure fast delivery.
Our highly trained Technical Support team provides comprehensive product support and is dedicated to resolving your issues quickly and efficiently.
Pundir et al., The Novel Receptor C5aR2 Is Required for C5a-Mediated Human Mast Cell Adhesion, Migration, and Proinflammatory Mediator Production. J. Immunol., Sep 2015; 195: 2774 - 2787.
Nonaka et al., Synthetic di-sulfated iduronic acid attenuates asthmatic response by blocking T-cell recruitment to inflammatory sites. PNAS, Jun 2014; 111: 8173 - 8178.
Lance, R. et. al. Gelatinase Contributes to the Pathogenesis of Endocarditis Caused by Enterococcus faecalis. Infect. Immun., Nov 2010; 78: 4936 - 4943.
Thurlow, L. et al. Gelatinase Contributes to the Pathogenesis of Endocarditis Caused by Enterococcus faecalis. Infect. Immun., 2010; 10.1128/IAI.01118-09.
For more citations of this product click here