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C-Peptide (human/mouse/rat) EIA Kit

Sensitive, Colorimetric Assay
Catalog #: K4757
$730.00

Product Details

Cat # +Size K4757-100
Size 100 assays
Detection Method Absorbance (450 nm)
Species Reactivity human, mouse, rat
Applications Quantitative protein detection, establishing normal range, validation of antibody array results.
Features & Benefits • Easy, convenient and time-saving method to assay C-Peptide.
• The minimum detectable concentration of C-Peptide is typically less than 772 pg/ml.
• This EIA kit shows no cross-reactivity with the following cytokines tested: e.g., Ghrelin, Nesfatin, Angiotensin II, NPY and APC.
Kit Components • C-Peptide Microplate
• Wash Buffer Concentrate (20x)
• Standard C-Peptide peptide
• Anti- C-Peptide polyclonal antibody
• Assay Diluent A
• Assay Diluent B
• Biotinylated C-Peptide peptide
• HRP-Streptavidin concentrate
• Positive control
• TMB One-Step Substrate Reagent
• Stop Solution
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

BioVision’s C-Peptide Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting C-Peptide peptide based on the principle of Competitive Enzyme Immunoassay. The microplate in the kit is pre-coated with anti-rabbit secondary antibody. After a blocking step and incubation of the plate with anti- C-Peptide antibody, both biotinylated C-Peptide peptide and peptide standard or targeted peptide in samples interacts competitively with the C-Peptide antibody. Uncompeted (bound) biotinylated C-Peptide peptide then interacts with Streptavidin-horseradish peroxidase (SAHRP), which catalyzes a color development reaction. The intensity of colorimetric signal is directly proportional to the amount of biotinylated peptide-SA-HRP complex and inversely proportional to the amount of C-Peptide peptide in the standard or samples. This is due to the competitive binding to C-Peptide antibody between biotinylated C-Peptide peptide and peptides in standard or samples. A standard curve of known concentration of C-Peptide peptide can be established and the concentration of C-Peptide peptide in the samples can be calculated accordingly. EIA-CPE-1 only detects C-peptide, not Insulin A or B chain. The minimum detectable concentration of C-Peptide is 772 pg/ml. The detection range for the kit is 0.1-1,000 ng/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.


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