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c-Jun Antibody

based on 1 citations in multiple journalsc-Jun Antibody14.1 4
Catalog #: 3702
$265.00

Product Details

Cat # +Size 3702-100
Size 100 µg
Antibody Target c-Jun
Host Rabbit
Antibody Type Polyclonal
Isotype Rabbit IgG
Immunogen Recombinant human c-Jun (1-79)-GST fution protein
Accession # AAH09874
Gene ID 3725
Appearance Colorless liquid
Concentration 0.5 mg/ml
Formulation 100 µg (0.5 mg/ml) affinity purified rabbit anti-c-Jun polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.
Purification Affinity purified
Species Reactivity Human, mouse, rat
Application Western blot, Immunohistochemistry
Application & Usage Western blotting (0.5-2 µg/ml) and Immunohistochemistry (20-40 µg/ml). However, the optimal conditions should be determined individually. The antibody only recognizes c-Jun, and will not detect the endogenous levels of Jun B or Jun D.
Handling The antibody solution should be gently mixed before use.
Storage Conditions -20 °C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Extracellular stimuli such as growth factors and UV irradiation stimulate phosphorylation of c-Jun at Ser63/73 and activate c-Jun dependent transcription. Mutation at Ser63/73 renders c-Jun nonresponsive to mitogenic and stress induced signaling pathways. JNK (c-Jun N-terminal Kinase) binds to the N-terminal region of c-Jun and phosphorylates c-Jun at Ser63/73. The activity of JNK is stimulated by the same signals that activate c-Jun.


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Yanamadala V et al. (2007) J. Biol. Chem. 282: 24352-24363.
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