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c-Fos Transcription Factor Activity Assay Kit (Colorimetric)

A 96-well plate based colorimetric assay to measure the activation of human c-Fos in nuclear extracts or cell lysates
Catalog #: K2100
$695.00

Product Details

Cat # +Size K2100-100
Size 100 assays
Detection Method Absorbance at 450 nm
Species Reactivity Human
Applications Semi-quantitative measurement of activation of human c-Fos in nuclear extracts or cell lysates
Features & Benefits The kit offers an easy, rapid, sensitive and non-radioactive way to detect the activation of c-Fos in samples.
Kit Components ● Plate Coated with DNA Probes
● Non-Competitor Oligo
● TMB Substrate
● Stop Solution
● Positive Control
● Plate Sealing Film
● Binding Buffer (5X)
● DTT (100 mM)
● Protease Inhibitor Cocktail
● c-Fos Primary Antibody
● Antibody Diluent Buffer
● HRP Conjugate Stock
● Wash Buffer (10X)
● Competitor Oligo
Storage Conditions Multiple Temperatures
Shipping Conditions Dry Ice
USAGE For Research Use Only! Not For Use in Humans.

Details

Activator protein-1 (aka activating protein 1, AP-1) is a critical transcription factor that plays a central role in the regulation of genes important in many cellular processes including cell proliferation, differentiation, apoptosis , survial, migration etc. AP-1 family is composed of homodimers and heterodimers of Jun, Fos, ATF or MAF protein families, which are characterized by the highly conserved dimeric basic leucine zipper (bZIP) DNA-binding domains. AP-1 protein is primarily regulated at the level of both Jun and Fos gene transcription involving MAPKs pathways and by post-translational modification via phosphorylation and dephosphorylation. AP-1 activity is induced by either physiological stimuli or environmental insults, including TPA, growth factors, polypeptide hormones, cytokines, UV irradiation, bacterial or viral infections etc. BioVision’s c-Fos Transcription Factor Activity Assay is a 96-well plate based colorimetric assay to measure the activation of human c-Fos in nuclear extracts or cell lysates. The kit offers an easy, rapid, sensitive and non-radioactive way to detect the activation of c-Fos in samples. In this assay, double stranded DNA sequence containing the c-Fos consensus binding site is coated on the 96-well plate. Active c-Fos in the cell lysate or the nuclear extract binds to the oligonucleotides on the plate. After the addition of c-Fos primary antibody that recognizes the c-Fos-oligonucleotide complex, a HRP-conjugated secondary antibody is added followed by the addition of TMB substrate and a color signal is developed, which is measured at 450 nm.


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