Butyrylcholinesterase Inhibitor Screening Kit (Colorimetric) (ab289837)
Key features and details
- Assay type: Cell-based (qualitative)
- Detection method: Colorimetric
- Platform: Microplate reader
Overview
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Product name
Butyrylcholinesterase Inhibitor Screening Kit (Colorimetric)
See all Butyrylcholinesterase kits -
Detection method
Colorimetric -
Assay type
Cell-based (qualitative) -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mammals -
Product overview
This Butyrylcholinesterase Inhibitor Screening Kit (ab289837, K2084) is a 96-well plate based, colorimetric assay to screen potential inhibitors of BChE. The kit utilizes the ability of active human BChE enzyme to hydrolyze the BChE substrate thereby generating a yellow colored product measured by absorbance at 412 nm. As the enzyme activity is inhibited in the presence of a potent, reversible BChE inhibitor, Rivastigmine, the color formation is inhibited. The assay is adapted for a 96-well plate format and provides a rapid, simple, and reliable test for high-throughput screening of BChE inhibitors.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests BChE Assay Buffer 1 x 50ml BChE Enzyme 1 vial BChE Probe Mix 1 vial BChE Substrate 1 x 100µl Rivastigmine (40 mM) 1 x 200µl -
Research areas
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Function
Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters. -
Tissue specificity
Detected in blood plasma (at protein level). Present in most cells except erythrocytes. -
Involvement in disease
Defects in BCHE are the cause of butyrylcholinesterase deficiency (BChE deficiency) [MIM:177400]. BChE deficiency is a metabolic disorder characterized by prolonged apnoea after the use of certain anesthetic drugs, including the muscle relaxants succinylcholine or mivacurium and other ester local anesthetics. The duration of the prolonged apnoea varies significantly depending on the extent of the enzyme deficiency. BChE deficiency is a multifactorial disorder. The hereditary condition is transmitted as an autosomal recessive trait. -
Sequence similarities
Belongs to the type-B carboxylesterase/lipase family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Acylcholine acylhydrolase
- BCHE
- Butyrylcholine esterase
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Images
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab289837 has not yet been referenced specifically in any publications.