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Brucella IgM ELISA Kit

An ELISA kit for the detection of IgM antibody to Brucella in human serum.

WARNING: This product can expose you to chemicals including TMB, which is [are] known to the State of California to cause cancer.  For more information go to www.P65Warnings.ca.gov.
Catalog #: E4710
$575.00

Product Details

Cat # +Size E4710-100
Size 96 assays
Detection Method Absorbance-450 nm
Species Reactivity Human
Applications This ELISA kit is used for in vitro quantitative determination of Brucella IgM in human serum samples
Kit Components • Positive Control
• Sample Diluent
• Microwell coated with Brucella antigen
• Negative Control
• Wash buffer (20X)
• Stop Solution
• Calibrator
• Enzyme conjugate
• TMB Substrate
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Brucella is a gram negative coccobacilli capable of infecting a wide range of animal and man. Of the three species causing human infection, B. melitensis is the most patogenic followed by B. suis and B. abortus . Brucellosis is transmitted through contaminated and untreated milk and milk products and by direct contact with infected animals (cattle, sheep, goats, pigs, camels, buffaloes, and, very recently, seals), animal carcasses, and abortion materials. BioVision's ELISA kit is for the detection of Brucella IgM antibody in human serum. Diluted patient serum (serum diluent contains sorbent to remove Rheumatoid Factor and human IgG interference) is added to wells coated with purified antigen. IgM specific antibody, if present, binds to the antigen. All unbound materials are washed away and the enzyme conjugate is added to bind to the antibody-antigen complex if present. Excess enzyme conjugate is washed off and the substrate is added. The plate is incubated to allow the hydrolysis of the substrate by the enzyme. The intensity of the color generated is proportional to the amount of IgM specific antibody in the sample.


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