Product Details
| abID | ab287841 |
|---|---|
| Detection Method | Absorbance (450 nm) |
| Species Reactivity | N/A |
| Applications | Detection and quantification of cell proliferation induced by growth factors, cytokines, mitogens, and nutrients. Analysis of cytotoxic and cytostatic compounds such as anticancer drugs, toxic agents and other pharmaceuticals. Determination of the inhibitory or stimulatory effects of various compounds on cell proliferation. |
| Features & Benefits | • Convenient & non-radioactive • High-throughput suitable • Highly-sensitive: detect as low as 50-100 proliferating cells. • Suitable for cell proliferation, chemotaxis and cytotoxicity |
| Kit Components | • BrdU (1000X) • Fixing/Denaturing Solution • BrdU Detection Antibody (300X) • Anti-mouse HRP-linked Antibody (2000X) • Antibody Diluent • Wash Buffer (10X) • TMB Substrate • Stop Solution |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
5-bromo-2-deoxyuridine (BrdU) is a pyrimidine analog. It gets incorporated into the newly synthesized DNA of proliferating cells in place of thymidine. Biovision's BrdU Cell Proliferation Assay Kit detects incorporated BrdU using a mouse anti-BrdU antibody. An anti-mouse HRP-linked secondary antibody is used to detect the anti-BrdU antibody bound to BrdU, which is followed by addition of TMB (a HRP substrate). The extent of color development is proportional to the quantity of BrdU incorporated into the cells and can be used directly as an indicator of cell proliferation. Compared to other cell proliferation assays, this kit detects only the proliferating cells and not the seeded cells. This highly sensitive, non-radioactive kit detects as less as 50-100 proliferating cells.
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My data shows huge variations. What could be the reason?
Huge variations can be caused by the nature of the cells. For example, increment in cell numbers following lymphocyte stimulation can lead to cluster formation, this effect can disturb the antibody recognition and thereby underestimate the response.
What is causing a high background in my samples?
High background is cause by insufficient washing. Increase the number of washing steps or prolong incubation with wash buffer upto 5 min per rinse.
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact yopur regional BioVision distributor.
What is the shelf life of this kit?
BioVisions's assay kits expire 6 months from the date of shipment. Some components of the kit may expire sooner as mentioned in the data sheet
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results
Gal Mazor, The lncRNA TP73-AS1 is linked to aggressiveness in glioblastoma and promotes temozolomide resistance in glioblastoma cancer stem cells. Cell Death Dis, March 2019; 30867410.
Ye Chen, Bromodomain and extraterminal proteins foster the core transcriptional regulatory programs and confer vulnerability in liposarcoma. Nat Commun., March 2019; 30903020.
Severa Bunda, CIC protein instability contributes to tumorigenesis in glioblastoma. Nat Commun., Feb 2019; 30737375.
Chen, Si-Le et al. (2016) Targeting CRMP‑4 by lentivirus‑mediated RNA interference inhibits SW480 cell proliferation and colorectal cancer growth, Exp Ther Med. 2016 Oct;12(4):2003-2008.
For more citations of this product click here
