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BMP-6 (Mouse) ELISA Kit

A highly sensitive sandwich ELISA kit for the quantitative measurement of BMP-6

WARNING: This product can expose you to chemicals including Sulfuric acid and TMB, which are known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov.
Catalog #: E5096
$725.00

Product Details

Cat # +Size E5096-100
Size 96 assays
Detection Method Absorbance is measured at 450 nm
Species Reactivity Mouse
Applications The ELISA kit is used for the quantitative detection of BMP-6 in serum, plasma, tissue homogenates and other biological fluids
Features & Benefits ● Highly sensitive and specific
● Assay Precision; Intra-Assay CV < 8% and Inter-Assay < 10%
● Recovery Rate: 87-98% for serum, 85-102% for EDTA plasma and 86-96% for heparin plasma
● Detection range: 78.125-5000 pg/ml
● Sensitivity: 46.875 pg/ml
Kit Components ● ELISA Microplate
● Wash Buffer (25X)
● Plate Sealers
● Lyophilized Standard (5000 pg)
● Sample/Standard Dilution Buffer
● Biotin-labeled Antibody (Concentrated)
● Antibody Dilution Buffer
● HRP-Streptavidin Conjugate (SABC)
● SABC Dilution Buffer
● TMB Substrate
● Stop Solution
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Bone morphogenetic protein 6 (BMP-6) belongs to the transforming growth factor-β (TGF-β) signaling family and has a very broad biological role in development. It plays an essential role in many developmental processes, including neurogenesis, vascular development, angiogenesis and osteogenesis. BMP-6 is able to induce all osteogenic markers in mesenchymal stem cells. It plays also an important role in the regulation of iron metabolism by acting as a ligand for hemojuvelin/HJV. It initiates the canonical BMP signaling cascade by associating with type I receptor ACVR1 and type II receptor ACVR2B. In turn, ACVR1 propagates signal by phosphorylating SMAD1/5/8 that travel to the nucleus and act as activators and repressors of transcription of target. BMP-6 has a role in chondroid formation, and also tubulo-glandular differentiation in pleomorphic adenomas. BioVision’s BMP-6 (Mouse) ELISA Kit is based on the Sandwich-ELISA principle. The micro-ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse BMP-6. Standards or samples are added to the micro-ELISA plate wells that bind to the specific antibody. Then a biotinylated detection antibody specific for Mouse BMP-6 and Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. The wells are washed, a TMB substrate solution is added to the wells and blue color develops in proportion to the amount of BMP-6 bound. The enzyme-substrate reaction is terminated by the addition of stop solution and turns color. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Mouse BMP-6. The concentration of Mouse BMP-6 in the samples can be calculated by comparing the OD of the samples to the standard curve.


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