Product Details
| abID | ab51998 |
|---|---|
| Alternate Name | BMP4, ZYME, BMP2B, BMP2B1 |
| Gene Symbol | BMP 4 |
| Gene ID | 652 |
| Accession # | P12644 |
| Source | E. coli |
| Appearance | Lyophilized protein |
| Physical Form Description | Sterile filtered and lyophilized powder |
| Molecular Weight | 13.0 kDa |
| Purity by SDS-PAGE | ≥98% |
| Purity by | ≥98% |
| Endotoxin Level | <0.1 ng/μg |
| Biological Activity | BioVision’s recombinant human BMP-4 is biologically active. The ED₅₀ as determined by dose-dependent induction of alkaline phosphatase activity with C2C12 cells is in the range of 2-12 µg/ml. |
| Reconstitution Instructions | Reconstitute to a concentration of 0.1-1.0 mg/ml in sterile 20 mM acetic acid. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use. |
| Handling | Centrifuge the vial prior to opening. |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not to be used in humans |
Details
BMPs (bone morphogenetic proteins) belong to the TGF-β superfamily of structurally related signaling proteins. As implied by their name, BMPs promote and regulate bone development, growth, remodeling and repair, in both prenatal development and postnatal growth of eye, heart, kidney, skin, and other tissues. Recombinant human BMP-4 produced from E. coli is a 13 kDa monomeric, non-glycosylated, polypeptide chain containing 116 amino acid residues.
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Riesco et al., In Vitro Generation of Zebrafish PGC-Like Cells. Biol Reprod, Nov 2014; 91: 114.
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