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BMP-1 (Mouse) ELISA Kit

A highly sensitive sandwich ELISA kit for the quantitative measurement of BMP-1

WARNING: This product can expose you to chemicals including Sulfuric acid and TMB, which are known to the State of California to cause cancer. For more information go to
Catalog #: E5095

Product Details

Cat # +Size E5095-100
Size 96 assays
Detection Method Absorbance is measured at 450 nm
Species Reactivity Mouse
Applications The ELISA kit is used for the quantitative detection of BMP-1 in serum, plasma, tissue homogenates and other biological fluids
Features & Benefits ● Recovery Rate: 90-105% for serum, 86-102% for EDTA plasma and 87-105% for heparin plasma
● Assay Precision; Intra-Assay CV < 8% and Inter-Assay < 10%
● Highly sensitive and specific
● Detection range: 78.125-5000 pg/ml
● Sensitivity: 46.875 pg/ml
Kit Components ● ELISA Microplate
● Wash Buffer (25X)
● Plate Sealers
● Lyophilized Standard (5000 pg)
● Sample/Standard Dilution Buffer
● Biotin-labeled Antibody (Concentrated)
● Antibody Dilution Buffer
● HRP-Streptavidin Conjugate (SABC)
● SABC Dilution Buffer
● TMB Substrate
● Stop Solution
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Bone morphogenetic protein 1 (BMP-1) belongs to the peptidase M12A family of bone morphogenetic proteins. It induces bone and cartilage development. BMP-1 is a metalloprotease that plays an Important role in regulating the formation of the extracellular matrix via processing of various precursor proteins into mature functional enzymes or structural proteins. Thereby participates in several developmental and physiological processes such as cartilage and bone formation, muscle growth and homeostasis, wound healing and tissue repair. Overexpression of BMP-1 can promote the osteogenesis of Background osteogenesis of bone marrow mesenchymal stem cells and provides an improved method of cell-based tissue engineering. BioVision’s BMP-1 (Mouse) ELISA Kit is based on the Sandwich-ELISA principle. The micro-ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse BMP-1. Standards or samples are added to the micro-ELISA plate wells that bind to the specific antibody. Then a biotinylated detection antibody specific for Mouse BMP-1 and Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. The wells are washed, a TMB substrate solution is added to the wells and blue color develops in proportion to the amount of BMP-1 bound. The enzyme-substrate reaction is terminated by the addition of stop solution and turns color. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Mouse BMP-1. The concentration of Mouse BMP-1 in the samples can be calculated by comparing the OD of the samples to the standard curve.

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