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bisBenzimide H 33342

A cell-permeable fluorescent DNA stain
Catalog #: 2179
SKU-Size Size Price Qty
2179-25 25 mg
2179-100 100 mg
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Product Details

Alternate Name 2′-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi-1H-benzimidazole trihydrochloride, HOE 33342, Hoechst 33342, bisBenzimide
Appearance Yellow solid
CAS # 23491-52-3
Molecular Formula C₂₇H₂₈N₆O · 3HCl
Molecular Weight 561.93
Purity ≥98% by HPLC
Solubility H₂O (~ 50 mg/ml)
InChi InChI=1S/C27H28N6O.3ClH/c1-3-34-21-8-4-18(5-9-21)26-28-22-10-6-19(16-24(22)30-26)27-29-23-11-7-20(17-25(23)31-27)33-14-12-32(2)13-15-33;;;/h4-11,16-17H,3,12-15H2,1-2H3,(H,28,30)(H,29,31);3*1H
PubChem CID 16760503
MDL Number MFCD00012678
Handling Protect from light and moisture
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


A cell-permeable fluorescent DNA stain with low cytotoxicity that binds preferentially to adenine-thymine (A-T) regions of DNA. This stain binds into the minor groove of DNA and exhibits distinct fluorescence emission spectra that are dependent on dye:base pair ratios. May be used for fluorescence microscopy or flow cytometry.

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