Recombinant human PD1 protein (Active) (Biotin) (ab286026)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, ELISA, Functional Studies, Size Exclusion Chromatography
Description
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Product name
Recombinant human PD1 protein (Active) (Biotin)
See all PD1 proteins and peptides -
Biological activity
Measured by its binding ability to bind Human PD-L1 in a functional ELISA with a linear range of 0.125-1 µg/ml.
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Purity
> 90 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
32 kDa -
Actual molecular weight
20 kDa -
Amino acids
25 to 165 -
Modifications
biotinylated -
Tags
His tag N-Terminus
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Conjugation
Biotin
Specifications
Our Abpromise guarantee covers the use of ab286026 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
ELISA
Functional Studies
Size Exclusion Chromatography
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Form
Liquid -
Additional notes
This product is manufactured by BioVision, an Abcam company and was previously called P1038 Biotinylated PD-1/PDCD1, Human Recombinant. P1038-50 is the same size as the 50 µg size of ab286026.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
Constituents: 20% Glycerol (glycerin, glycerine), 80% PBS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- CD279
- CD279 antigen
- hPD 1
see all -
Function
Possible cell death inducer, in association with other factors. -
Involvement in disease
Genetic variation in PDCD1 is associated with susceptibility to systemic lupus erythematosus type 2 (SLEB2) [MIM:605218]. Systemic lupus erythematosus is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Developmental stage
Induced at programmed cell death. -
Cellular localization
Membrane. - Information by UniProt
Images
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SDS-PAGE (4-20%) analysis of ab286026, with a MW of 19.7kDa.
M: Protein Marker
1: Biotinylated Human PD-1 (10 μg)
2: Biotinylated Human PD-1 (5 μg)
3: Biotinylated Human PD-1 (2 μg)
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ab286026 is able to bind Human PD-L1 immobilized at 2 µg/ml in a functional ELISA (n = 3)
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Size exclusion chromatography analysis of ab286026 using a Superose 12 column at 0.4 ml/min in 50 mM sodium phosphate, 300 mM NaCl, pH 7.5
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab286026 has not yet been referenced specifically in any publications.