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Biotin Ligase (BirA), Recombinant

An enzyme that transfers biotin to biotin-accepting proteins
Catalog #: P1674
$295.00

Product Details

Cat # +Size P1674-100
Size 100 µg
Alternate Name BirA, Biotin operon repressor protein; Biotin holoenzyme synthetase; biotin-(acetyl-CoA carboxylase) synthetase
Gene ID 948469
Accession # P06709
Source E.Coli
Appearance Sterile Filtered colorless solution
Physical Form Description Liquid
Molecular Weight 37.5 kDa (with N-terminal His tag)
Purity by SDS-PAGE ≥ 95%
Formulation In 50 mM Tris (pH 7.5), 50 mM NaCl, 3 mM DTT, 30% glycerol
Amino Acid Sequence Amino acids 2 to 321
Specific Activity ≥ 30 mU/mg
Unit Definition One unit of BirA is defined as the amount of enzyme that catalyzes the biotinylation of 1 µmol of Avi-tagged protein (CD40L-Avi) per minute under the assay conditions, where 50% of CD40L-Avi is biotinylated in 60 min at 30 °C in the reaction buffer contai
Handling Centrifuge the vial prior to opening.
Storage Conditions -80 °C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Biotin-protein ligase (EC 6.3.4.15) activates biotin to form biotinyl 5'-adenylate and transfers the biotin to biotin-accepting proteins. BirA is E coli biotin ligase that biotinylates specifically a lysine side chain within a 15-amino acid acceptor peptide (also known as Avi-tag).


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