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BioSim™ Vedolizumab (Entyvio®)(Human) ELISA Kit

This ELISA kit is used for in vitro quantitative determination of Vedolizumab.
Catalog #: E4697

In stock


Product Details

Cat # +Size E4697-100
Size 96 assays
Detection Method colorimetric
Species Reactivity Human
Applications • Sensitivity: 30 ng/ml
• Recovery rate: 85 – 115% with normal human serum samples with known concentrations
• Detection Range: 30 - 1000 ng/ml
• This ELISA kit is used for in vitro quantitative determination of Vedolizumab
• Cross Reactivity:
Features & Benefits • Detection Range: 30 - 1000 ng/ml
• Cross Reactivity: Except for Vedolizumab, there is no cross reaction with other therapeutic antibodies and native serum immunoglobins.
• Sensitivity: 30 ng/ml,This ELISA kit is used for in vitro quantitative determination of Vedolizumab
Kit Components • Vedolizumab Standards
• Wash buffer (20X)
• Plate sealers
• Micro ELISA Plate
• HRP-conjugate Probe
• Stop Solution
• Assay Buffer
• TMB substrate (Avoid light)
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Vedolizumab is a recombinant humanized IgG1 monoclonal antibody directed against the human lymphocyte α4β7 integrin, a key mediator of gastrointestinal inflammation. It is used in the treatment of moderate to severe active ulcerative colitis and Crohn's disease for patients who have had an inadequate response with, lost response to, or were intolerant to inhibitors of tumor necrosis factor-alpha (TNF-alpha) or other conventional therapies. BioSim™ Vedolizumab ELISA kit is a Solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. Standards and samples (serum or plasma) are incubated in the microtitre plate coated with the reactant for Vedolizumab (Cyramza®). After incubation, the wells are washed. A horse radish peroxidase (HRP) conjugated probe is added and binds to Vedolizumab captured by the reactant on the surface of the wells. Following incubation wells are washed and the bound enzymatic activity is detected by addition of chromogen-substrate. The color developed is proportional to the amount of Vedolizumab in the sample or standard.

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