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BioSim™ Ustekinumab (Stelara®) (Human) ELISA Kit

A Sandwich ELISA kit for the qualitative detection of ustekinumab (Stelara) in human serum or plasma.

WARNING: This product can expose you to chemicals including TMB, which is [are] known to the State of California to cause cancer.  For more information go to
Catalog #: E4695

Product Details

Cat # +Size E4695-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Human
Applications • Sensitivity: 3 ng/ml
• Detection Range: 3 - 100 ng/ml
• This ELISA kit is used for in vitro quantitative determination of Ustekinumab
• Recovery rate: 85 – 115% with normal human serum samples with known concentrations
• Cross Reactivity: No
Features & Benefits • Sensitivity: 30 ng/ml
• Easy, convenient, sensitive and time-saving method to measure the level of Ustekinumab in human serum and plasma.
• Recovery rate: 85 – 115% with normal human serum samples with known concentrations
• Assay Precision: Intra-Assay: CV < 15%; Inter-Assay: CV < 15% (CV (%) = SD/mean X 100)
• Detection Range: 30 - 1000 ng/ml
• Cross Reactivity: No significant cross-reactivity or interference with other proteins present in native human serum or other therapeutic immunoglobulins.
Kit Components • Plate Sealer
• Wash Buffer (20X)
• Standards
• Microtiter Plate
• Stop Solution
• HRP-Conjugate
• Assay Buffer
• TMB Substrate
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Ustekinumab is a fully human monoclonal antibody that binds with high specificity and affinity to the cytokines interleukin (IL)-12 and IL-23, thereby suppressing IL-12- and IL-23-mediated inflammation associated with psoriasis. Ustekinumab (STELARA®) is the only FDA-approved medicine that targets IL-12 and IL-23, which are thought to be associated with gastrointestinal inflammation in Crohn's disease. BioSim Ustekinumab (Stelara®) ELISA Kit is a sandwich based ELISA kit. Standards and samples are incubated in the microtitre plate coated with the reactant for Ustekinumab (Stelara®). After incubation, the wells are washed. HRP-conjugated probe is added and binds to ustekinumab captured by the reactant on the surface of the wells. Following incubation wells are washed and the bound enzymatic activity is detected by addition of chromogen-substrate. The color developed is proportional to the amount of ustekinumab in the sample or standard. Results of samples can be determined directly using the standard curve.

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