BioSim™ Tocilizumab (Human) ELISA Kit

This ELISA kit is used for in vitro quantitative determination of Tocilizumab in human serum and plasma samples
Catalog #: E4858 | abID: ab282910

Product Details

abID ab282910
Cat # +Size E4858-100
Size 96 assays
Detection Method Sandwich ELISA, Absorbance (450 nm)
Species Reactivity Human
Applications This ELISA kit is used for in vitro quantitative determination of Tocilizumab
Features & Benefits • Recovery rate: < 100 ± 30% with normal human serum samples with known concentrations
• Assay Precision: Intra-Assay: CV < 30%; Inter-Assay: CV < 30% (CV (%) = SD/mean X 100)
• Detection Range: 10 - 300 ng/ml
• This ELISA kit is used for in vitro quantitative determination of Tocilizumab
• Sensitivity: 10 ng/ml
• Cross Reactivity: Except for Tocilizumab, there is no cross reaction with other therapeutic antibodies and native serum immunoglobins.
Kit Components • Micro ELISA Plate
• Tocilizumab Standards (S1 – S7)
• Assay Buffer (5X)
• HRP-conjugate Probe
• TMB substrate (Avoid light)
• Stop Solution
• Wash buffer (20X)
• Plate sealers
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Tocilizumab is a recombinant, humanized, anti-human interleukin 6 (IL-6) receptor monoclonal antibody. The light chain is made up of 214 amino acids. The heavy chain is made up of 448 amino acids. The four polypeptide chains are linked intra- and intermolecularly by disulfide bonds. BioSim™ Tocilizumab (Human) ELISA Kit has been developed for specific quantification of Tocilizumab concentration in human serum or plasma with high sensitivity and reproducibility. Tocilizumab ELISA kit is based on the sandwich ELISA principle. Standards and samples (serum or plasma) are added in the microtiter plate coated with the reactant for Tocilizumab. After incubation, the wells are washed. The HRP conjugated probe is added and binds to Tocilizumab captured by the reactant on the surface of the wells. Following incubation wells are washed and the bound enzymatic activity is detected by addition of TMB chromogen substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the amount of Tocilizumab in the sample or standard. Results of samples can be determined directly using the standard curve.


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